Dynamic responses of germination characteristics and antioxidant systems to alfalfa (Medicago sativa) seed aging based on transcriptome.

Seed aging poses a significant challenge to agronomic production and germplasm conservation. Reactive oxygen species (ROS) are highly involved in the aging process. However, dynamic response of germination characteristics and antioxidant system to seed aging are not yet very clear.

Telomerase reverse transcriptase, a telomere length maintenance protein in alfalfa (Medicago sativa), confers Arabidopsis thaliana seeds aging tolerance via modulation of telomere length.

Numerous studies have investigated seed aging, with a particular emphasis on the involvement of reactive oxygen species. Reactive oxygen species diffuse into the nucleus and damage telomeres, resulting in loss of genetic integrity. Telomerase reverse transcriptase (TERT) plays an essential role in maintaining plant genomic stability.

Overexpression of protection of telomeres 1 (POT1), a single-stranded DNA-binding proteins in alfalfa (Medicago sativa), enhances seed vigor.

Extensive bodies of research are dedicated to the study of seed aging with a particular focus on the roles of reactive oxygen species (ROS), and the ensuing oxidative damage during storage, as a primary cause of seed vigor decreasing. ROS diffuse to the nucleus and damage the telomeres, resulting in a loss of genetic integrity. Protection of telomeres 1 (POT1) is a telomeric protein that binds to the telomere region, and plays an essential role in maintaining genomic stability in plants.

Photosynthetic performance of glumes of oat spikelets is more stable for grain-filling stage under drought stress.

Drought stress affects plant photosynthesis, leading to a reduction in the quality and yield of crop production. Non-foliar organs play a complementary role in photosynthesis during plant growth and development and are important sources of energy. However, there are limited studies on the performance of non-foliar organs under drought stress.

Using Machine Learning Methods Combined with Vegetation Indices and Growth Indicators to Predict Seed Yield of .

Smooth bromegrass () is a perennial, high-quality forage grass. However, its seed yield is influenced by agronomic practices, climatic conditions, and the growing year. The rapid and effective prediction of seed yield can assist growers in making informed production decisions and reducing agricultural risks.

Genomic identification and expression profiling of WRKY genes in alfalfa (Medicago sativa) elucidate their responsiveness to seed vigor.

Background: Seed aging is a critical factor contributing to vigor loss, leading to delayed forage seed germination and seedling growth. Numerous studies have revealed the regulatory role of WRKY transcription factors in seed development, germination, and seed vigor. However, a comprehensive genome-wide analysis of WRKY genes in Zhongmu No.

Germination of aged oat seeds associated with changes in antioxidant enzyme activity and storage compounds mobilization.

Germination of aged seeds may be associated with specific metabolic changes. The objective of this study was to examine physiological and metabolic alterations before and after germination of control and aged oat (Avena sativa) seeds. The activity of antioxidant enzymes and the level of storage compounds were measured in the embryo and endosperm at 0, 4, 16, and 32 h of imbibition for control seeds and 0, 4, 16, 32, and 60 h of imbibition for medium vigor seeds after artificially accelerated aging; metabolomic changes were determined in embryos at 16 and 32 h of seed imbibition.

Genomic Identification and Expression Profiling of Lesion Simulating Disease Genes in Alfalfa () Elucidate Their Responsiveness to Seed Vigor.

Seed aging, a common physiological phenomenon during forage seed storage, is a crucial factor contributing to a loss of vigor, resulting in delayed seed germination and seedling growth, as well as limiting the production of hay. Extensive bodies of research are dedicated to the study of seed aging, with a particular focus on the role of the production and accumulation of reactive oxygen species (ROS) and the ensuing oxidative damage during storage as a primary cause of decreases in seed vigor. To preserve optimal seed vigor, ROS levels must be regulated.

Analysis of Gene Expression Profile in Alfalfa () Indicates Their Response to Abiotic Stress and Seed Aging.

Seed aging is always taken as a crucial factor for vigor loss due to delayed seed germination and seedling growth, which limits hay production. Many studies have found that telomeres are closely related to abiotic stress and seed vigor. However, the molecular mechanism of telomeres' response to abiotic stress, seed vigor, and the maintenance mechanism of plant telomere homeostasis still remain unclear.

Genome-wide analysis and expression profiling of glyoxalase gene families in oat () indicate their responses to abiotic stress during seed germination.

Abiotic stresses have deleterious effects on seed germination and seedling establishment, leading to significant crop yield losses. Adverse environmental conditions can cause the accumulation of methylglyoxal (MG) within plant cells, which can negatively impact plant growth and development. The glyoxalase system, which consists of the glutathione (GSH)-dependent enzymes glyoxalase I (GLX1) and glyoxalase II (GLX2), as well as the GSH-independent glyoxalase III (GLX3 or DJ-1), plays a crucial role in detoxifying MG.

Integrating optical imaging techniques for a novel approach to evaluate Siberian wild rye seed maturity.

Advances in optical imaging technology using rapid and non-destructive methods have led to improvements in the efficiency of seed quality detection. Accurately timing the harvest is crucial for maximizing the yield of higher-quality Siberian wild rye seeds by minimizing excessive shattering during harvesting. This research applied integrated optical imaging techniques and machine learning algorithms to develop different models for classifying Siberian wild rye seeds based on different maturity stages and grain positions.

Single Seed Identification in Three Species via Multispectral Imaging Combined with Stacking Ensemble Learning.

Multispectral imaging (MSI) has become a new fast and non-destructive detection method in seed identification. Previous research has usually focused on single models in MSI data analysis, which always employed all features and increased the risk to efficiency and that of system cost. In this study, we developed a stacking ensemble learning (SEL) model for successfully identifying a single seed of sickle alfalfa (), hybrid alfalfa (), and alfalfa ().

Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat () Indicate Their Responses to Abiotic Stress during Seed Imbibition.

Abiotic stress disturbs plant cellular redox homeostasis, inhibiting seed germination and plant growth. This is a crucial limitation to crop yield. Glutathione reductase (GR) is an important component of the ascorbate-glutathione (AsA-GSH) cycle which is involved in multiple plant metabolic processes.

Physiological and Molecular Responses of to Rust Infection.

Zoysiagrass () is a popular turfgrass species and is widely used for sport turf and urban landscape. Zoysiagrass is often infected by , which causes a loss in turf quality. The physiological and molecular mechanisms of rust resistance are poorly understood in this species.

Non-Destructive Testing of Alfalfa Seed Vigor Based on Multispectral Imaging Technology.

Seed vigor is an important index to evaluate seed quality in plant species. How to evaluate seed vigor quickly and accurately has always been a serious problem in the seed research field. As a new physical testing method, multispectral technology has many advantages such as high sensitivity and accuracy, nondestructive and rapid application having advantageous prospects in seed quality evaluation.

Dynamic Responses of Antioxidant and Glyoxalase Systems to Seed Aging Based on Full-Length Transcriptome in Oat ( L.).

Seed aging is a major challenge for food security, agronomic production, and germplasm conservation, and reactive oxygen species (ROS) and methylglyoxal (MG) are highly involved in the aging process. However, the regulatory mechanisms controlling the abundance of ROS and MG are not well characterized. To characterize dynamic response of antioxidant and glyoxalase systems during seed aging, oat ( L.

Non-Destructive Identification of Naturally Aged Alfalfa Seeds via Multispectral Imaging Analysis.

Seed aging detection and viable seed prediction are of great significance in alfalfa seed production, but traditional methods are disposable and destructive. Therefore, the establishment of a rapid and non-destructive seed screening method is necessary in seed industry and research. In this study, we used multispectral imaging technology to collect morphological features and spectral traits of aging alfalfa seeds with different storage years.

Genome-Wide Identification of GRAS Gene Family and Their Responses to Abiotic Stress in .

Alfalfa () is a high-quality legume forage crop worldwide, and alfalfa production is often threatened by abiotic environmental stresses. GRAS proteins are important transcription factors that play a vital role in plant development, as well as in response to environmental stress. In this study, the availability of alfalfa genome "Zhongmu No.

PSII Activity Was Inhibited at Flowering Stage with Developing Black Bracts of Oat.

The color of bracts generally turns yellow or black from green during cereal grain development. However, the impact of these phenotypic changes on photosynthetic physiology during black bract formation remains unclear. Two oat cultivars ( L.

Physiological and proteomic analysis reveals the impact of boron deficiency and surplus on alfalfa (Medicago sativa L.) reproductive organs.

Boron (B), an essential element for increasing seed yield and germinability in alfalfa (Medicago sativa L.), plays a vital role in its reproductive processes. However, effects of B stress on physiological and proteomic changes in reproductive organs related to alfalfa seed yield and germinability are poorly understood.

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat ( L.) Seeds.

Melatonin priming is an effective strategy to improve the germination of aged oat ( L.) seeds, but the mechanism involved in its time-course responses has remained largely unknown. In the present study, the phenotypic differences, ultrastructural changes, physiological characteristics, and proteomic profiles were examined in aged and melatonin-primed seed (with 10 μM melatonin treatment for 12, 24, and 36 h).

Identification and characterization of regulatory pathways involved in early flowering in the new leaves of alfalfa (Medicago sativa L.) by transcriptome analysis.

Background: Alfalfa (Medicago sativa L.) is a perennial legume extensively planted throughout the world as a high nutritive value livestock forage. Flowering time is an important agronomic trait that contributes to the production of alfalfa hay and seeds.

Conserved and Opposite Transcriptome Patterns during Germination in and .

Seed germination is a critical process for completion of the plant life cycle and for global food production. Comparing the germination transcriptomes of barley () to revealed the overall pattern was conserved in terms of functional gene ontology; however, many oppositely responsive orthologous genes were identified. Conserved processes included a set of approximately 6000 genes that peaked early in germination and were enriched in processes associated with RNA metabolism, e.