Fluxomic, Metabolomic, and Transcriptomic Analyses Reveal Metabolic Responses to Phenazine-1-carboxamide Synthesized in .

Phenazine-1-carboxamide (PCN) has been exploited as a successful biopesticide due to its broad-spectrum antifungal activity. We engineered a PCN-overproducing strain through overexpressing shikimate pathway genes (, , , and ) and deleting negative regulatory genes (, , and ). The optimized strain produced 1.