Human umbilical cord mesenchymal stem cells improve the ovarian function through oxidative stress-mediated PERK/eIF-2α/ATF4/CHOP signaling in premature ovarian insufficiency mice.

Background: Premature ovarian insufficiency (POI) is a refractory disease that severely affects female fertility. The PERK/eIF-2α/ATF4/CHOP pathway is one of the classical pathways involved in the unfolded protein response to endoplasmic reticulum stress by regulating protein synthesis and promoting apoptosis. This study aimed to investigate the functional role and mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) in the POI animal model through the PERK/eIF-2α/ATF4/CHOP pathway.

Monosaccharide composition, physicochemical characteristics, and prebiotic activity of purified polysaccharides from red mushroom prepared using ultrasonic-assisted extraction.

The objective of this study was to develop a purified polysaccharide for use in prebiotic research. The monosaccharide composition, physicochemical characteristics, and prebiotic activities of the polysaccharides were determined. Additionally, the total protein, total sugar, antioxidant activities, and molecular weights of the polysaccharides were subjected to analysis.

Effects of Professional Growth-Need Strength on Nursing Competencies: A Care-Task Design and Clinical Reasoning Program.

The study aimed to examine the relationship among professional growth-need strength, care-task characteristics, critical thinking, and holistic nursing competencies after a care-task design and clinical reasoning program intervention in nursing students. This study used a longitudinal study design. The study was conducted from August 12, 2019 to July 30, 2020.

The Impact of Clinical Practice Stress on Nursing Professional Competence among Undergraduate Nursing Students: A Cross-Sectional Study.

Aim: This study aims to explore the impact of undergraduate nursing students' clinical practice stress on nursing professional competence.

Methods: The research was performed as a cross-sectional study. From January 2023 to February 2023, a total of 114 nursing students in their first clinical practice at a university in Taiwan were included in the survey.

Effects of cooperative learning and situational simulation on nursing competence in clinical practice among nursing students: A quasi-experimental study.

Background: Appropriate cooperative learning and situational simulation designs can enhance mental health and nursing competence in clinical practice. This approach is crucial for cultivating the clinical nursing competence of nursing students.

Aim: To determine the effect of combining cooperative learning and situational simulation on nursing competence in clinical practice.

A rapid and reliable targeted LC-MS/MS method for quantitative analysis of the Tryptophan-NAD metabolic network disturbances in tissues and blood of sleep deprivation mice.

Background: TRY-NAD metabolic network includes TRY (tryptophan), 5-HT (5-hydroxytryptamine), KYN (kynurenine), and NAD (nicotinamide adenine dinucleotide) pathway, which plays a significant role in neurological diseases and ageing. It is important to monitor these metabolites for studying the pathological anatomy of disease and treatment of responses evaluation. Although previous studies have reported quantitative methods for several metabolites in the network, the bottlenecks of simultaneously quantifying the whole metabolic network are their similar structures, diverse physico-chemical properties, and instability.

Biosynthesis of Diverse Ephedra-Type Alkaloids via a Newly Identified Enzymatic Cascade.

Ephedra-type alkaloids represent a large class of natural and synthetic phenylpropanolamine molecules with great pharmaceutical values. However, the existing methods typically rely on chemical approaches to diversify the -group modification of Ephedra-type alkaloids. Herein, we report a 2-step enzymatic assembly line for creating structurally diverse Ephedra-type alkaloids to replace the conventional chemical modification steps.

NCAPD2 serves as a potential prognostic biomarker for lung adenocarcinoma and promotes cell proliferation, migration, invasion and cell cycle .

Objectives: The pro-oncogenic effects of NCAPD2 have been extensively studied across various tumor types; however, its precise role within the context of lung adenocarcinoma (LUAD) remains elusive. This study aims to elucidate the biological functions of NCAPD2 in LUAD and unravel the underlying mechanistic pathways.

Methods: Utilizing bioinformatics methodologies, we explored the differential expression of NCAPD2 between normal and tumor samples, along with its correlations with clinical-pathological characteristics, survival prognosis, and immune infiltration.

Molecular characterization of a catalase gene in the freshwater green alga Closterium ehrenbergii and its putative function against abiotic stresses.

Catalases (CATs) are ubiquitous antioxidant enzymes that prevent cellular oxidative damage through the decomposition of HO. However, there is relatively little information on CAT in the worldwide-distributed freshwater green alga Closterium ehrenbergii. Here, we cloned the full-length catalase cDNA from C.

RILPL2 as a potential biomarker for predicting enhanced T cell infiltration in non-small cell lung cancer.

Our previous bioinformatics analysis has revealed that Rab-interacting lysosomal protein-like 2 (RILPL2) is associated with tumor immune microenvironment in non-small cell lung cancer (NSCLC). In our study, we collected 140 patients with primary NSCLC to verify the RILPL2 expression and its prognostic value, the relationship between RILPL2 expression and CD4, CD8T cell infiltration. A total of 140 patients who had been diagnosed with primary NSCLC (including 66 lung adenocarcinomas and 74 lung squamous cell carcinomas) were enrolled in our study.

Widely targeted metabolomic analysis reveals effects of yellowing process time on the flavor of vine tea ().

The bitter and astringent taste and miscellaneous smell of vine tea prevent its further development. In this study, we used a processing technology that mimics yellow tea to improve the flavor of vine tea and revealed its internal reasons through metabolomics. Sensory evaluation showed the yellowing process for 6-12 h reduced the bitterness and astringency significantly, and enriched the aroma.

Role of NF-κB p65/TNF-α in Cell Apoptosis in the Fetal Membranes of Pregnant Women with Preterm Premature Rupture of Membranes.

Objective: This study aimed to investigate the roles of nuclear factor-kappa B p65 (NF-[Formula: see text]B p65) and tumor necrosis factor- (TNF-) in cell apoptosis occurring in the fetal membranes of pregnant women who experience preterm premature rupture of membranes (PPROM).

Methods: This was a case-control study involving 57 pregnant women who delivered in the obstetric department of Affiliated Loudi Hospital, Hengyang Medical School, University of South China, from June 2021 to June 2022. Samples of fetal membrane tissue were collected from pregnant women with PPROM (n=27) and pregnant women who had normal deliveries (control group; n=30).

Nuclear genome of dinoflagellates: Size variation and insights into evolutionary mechanisms.

Recent progress in high-throughput sequencing technologies has dramatically increased availability of genome data for prokaryotes and eukaryotes. Dinoflagellates have distinct chromosomes and a huge genome size, which make their genomic analysis complicated. Here, we reviewed the nuclear genomes of core dinoflagellates, focusing on the genome and cell size.

Neuropharmacological insights into Gardenia jasminoides Ellis: Harnessing therapeutic potential for central nervous system disorders.

Background: In China, Gardenia jasminoides Ellis (GJE) has a longstanding history of application. The Ministry of Health has listed it as one of the first pharmaceutical or food resources. In ethnic, traditional, and folk medicine, GJE has been used to treat fever and cold and relieve nervous anxiety.

The effect of vine tea (Ampelopsis grossedentata) extract on fatigue alleviation via improving muscle mass.

Ethnopharmacological Relevance: Vine Tea (VT, Ampelopsis grossedentata), boasts a venerable tradition in China, with a recorded consumption history exceeding 1200 years. Predominantly utilized by ethnic groups in southwest China, this herbal tea is celebrated for its multifaceted therapeutic attributes. Traditionally, VT has been employed to alleviate heat and remove toxins, exhibit anti-inflammatory properties, soothe sore throats, lower blood pressure, and fortify bones and muscles.

Comprehensive Analysis of Risk Factors for Bronchopulmonary Dysplasia in Preterm Infants in Taiwan: A Four-Year Study.

Bronchopulmonary dysplasia (BPD) is a major respiratory condition mainly affecting premature infants. Although its occurrence is global, risk factors may differ regionally. This study, involving 3111 infants with birth weight ≤ 1500 gm or gestational age (GA) < 30 weeks, aimed to identify risk factors for BPD and BPD/mortality in Taiwan using data from the Taiwan Neonatal Network.

Predictors of Teaching Self-efficacy in Clinical Nursing Teachers: A Cross-sectional Study.

Aim: This study aimed to investigate the relationship among clinical nursing teachers' perceived stress, job stress, and teaching self-efficacy and to identify important predictors of clinical nursing teachers' teaching self-efficacy.

Method: A cross-sectional study was designed. Data were collected from May 2019 to January 2020, and 205 clinical nursing teachers were recruited from the Department of Nursing in Taiwan.

Transfer Behaviors of 30 Pesticide Residues during the Common Processing of Ginseng.

To reduce safety issues of ginseng products caused by excessive pesticide levels, this paper systematically elucidates the transfer rules of pesticides during processing by calculating the transfer rates and processing factors (PFs). The common methods of ginseng processing (water extraction, alcohol extraction, concentration, water extraction and alcohol precipitation, vacuum drying, freeze drying, spray drying) and 30 pesticides frequently occurring in ginseng were used as research objects. The results indicate that the pesticide residues in alcohol extract are much higher than those in water extract.

Prognostic value of RILPL2 and its correlation with tumor immune microenvironment and glycolysis in non-small cell lung cancer.

Rab-interacting lysosomal protein - like 2 (RILPL2) has been reported to be associated with prognosis and tumor biological functions in breast cancer and endometrial carcinoma. However, its expression and functional role in non-small cell lung cancer (NSCLC) remain unclear. The expression and clinical data of lung adenocarcinoma (LUAD) and lung squamous carcinoma (LUSC) were downloaded from the TCGA database.

Trace heavy metals and harmful elements in roots and rhizomes of herbs: Screening level analysis and health risk assessment.

Objective: Heavy metal and harmful element contamination are frequently reported in Chinese herbal medicines (CHMs), and roots and rhizomes parts showed a higher content than other parts. To investigate the residue level and assess the potential human health risk of heavy metals and harmful elements in roots and rhizomes, 720 batches of the sample representing 20 species of herbs from different sources were collected.

Methods: The content of Pb, Cd, As, Hg, and Cu in the digests was determined using ICP-MS The chronic hazard index estimate based on non-cancer hazard quotient (HQ) was applied for potential health risk assessment of Pb, Cd, As, Hg, and Cu via consumption of CHMs.

Development of shuttle vectors for rapid prototyping of engineered Synechococcus sp. PCC7002.

Cyanobacteria are of particular interest for chemical production as they can assimilate CO and use solar energy to power chemical synthesis. However, unlike the model microorganism of Escherichia coli, the availability of genetic toolboxes for rapid proof-of-concept studies in cyanobacteria is generally lacking. In this study, we first characterized a set of promoters to efficiently drive gene expressions in the marine cyanobacterium Synechococcus sp.

Factors associated with facial pressure injury in patients receiving non-invasive positive pressure ventilation mask: A retrospective case-control study.

Aims And Objectives: This study aimed to investigate factors associated with facial pressure injury (FPI) in patients receiving non-invasive positive pressure ventilation (NIPPV) during hospitalisation in the intensive care unit (ICU) and to identify predictors of FPI.

Background: Non-invasive positive pressure ventilation is a method of treating patients with acute and chronic respiratory failure. However, FPI may occur due to unsuitable nasal-oral NIPPV masks and discomfort in contact with the skin surface.

Evaluation of Effecting Factors About Exercise Motivation in Patients with Parkinson's Disease.

Aim: The aim of this study was to explore the factors that affect exercise motivation in patients with Parkinson's disease.

Methods: Data collected in Taiwan from March 2016 to January 2017. Using convenience sampling, a total of 49 participants were recruited, and participants who usually regularly exercise ≥ 150 minutes per week.