In situ identification of gastroprotective components from Dendrobium huoshanense using magnetic nanoparticles combined with biomaterials assisted EFISI-MS.

The identification of natural active compounds is typically a cumbersome and time-intensive process due to the necessity for elaborate sample pretreatment, leading to inefficiencies. Although recent advancements have introduced methods for targeted screening of these active compounds, they still demand intricate sample handling, and in severe cases, critical active compounds may be lost or degraded beyond detection. This study capitalizes on the novel discovery that FeO magnetic nanoparticles (MNPs) can be stably immobilized onto a magnetized steel probe of the electrostatic field induced spray ionization mass spectrometry (EFISI-MS) platform.

SDDSynergy: Learning Important Molecular Substructures for Explainable Anticancer Drug Synergy Prediction.

Drug combination therapies are well-established strategies for the treatment of cancer with low toxicity and fewer adverse effects. Computational drug synergy prediction approaches can accelerate the discovery of novel combination therapies, but the existing methods do not explicitly consider the key role of important substructures in producing synergistic effects. To this end, we propose a significant substructure-aware anticancer drug synergy prediction method, named SDDSynergy, to adaptively identify critical functional groups in drug synergy.

Structurally Diverse Bisbenzylisoquinoline Alkaloids with Antiadipogenic Activity through Downregulation from the Embryo of Seeds.

Six undescribed and six known bisbenzylisoquinoline alkaloids were isolated from the embryo of seeds. Their structures were fully characterized by a combination of H, C NMR, 2D NMR, and HRESIMS analyses, as well as ECD computational calculations. The antiadipogenic activity of 11 alkaloids was observed in a dose-responsive manner, leading to the suppression of lipid accumulation in 3T3-L1 cells.

Ultra-performance liquid chromatography-quadrupole time-of-flight mass combined with UNIFI to study the mechanism of Tao Hong Si Wu Decoction in the treatment of postpartum blood stasis.

Postpartum hemorrhage can lead to a variety of maternal complications. Tao Hong Si Wu Decoction (THSWD) is a traditional Chinese medicine used for treating gynecological diseases. However, the active ingredients of THSWD and its pharmacological mechanism of treatment for postpartum blood stasis still remained unclear.

B-LBConA: a medical entity disambiguation model based on Bio-LinkBERT and context-aware mechanism.

Background: The main task of medical entity disambiguation is to link mentions, such as diseases, drugs, or complications, to standard entities in the target knowledge base. To our knowledge, models based on Bidirectional Encoder Representations from Transformers (BERT) have achieved good results in this task. Unfortunately, these models only consider text in the current document, fail to capture dependencies with other documents, and lack sufficient mining of hidden information in contextual texts.

Swallowing function after acute ischemic stroke: Development and validation of a novel clinical prognostic model.

Background: Predicting the duration of dysphagia after acute ischemic stroke (AIS) is important for clinical treatment decisions.

Objective: The purpose of this study is to assess the swallowing function of AIS patients and to develop and validate a prognostic model for the need for nasogastric tube (NGT) in these patients.

Materials And Methods: We included 554 AIS patients during 2018-2019 as the development group and had 186 AIS patients as the external validation group.

OsWHY1 Interacts with OsTRX z and is Essential for Early Chloroplast Development in Rice.

WHIRLY (WHY) family proteins, a small family of single-stranded DNA (ssDNA) binding proteins, are widely found in plants and have multiple functions to regulate plant growth and development. However, WHY in rice has received less attention. In this study, we continued our previous study on OsTRX z that is important for chloroplast development.

Rapid separation and characterization of the in vitro metabolites of moscatilin by ultra-high performance liquid chromatography coupled to hybrid quadrupole orbitrap tandem mass spectrometry.

Moscatilin, a bioactive ingredient isolated from Dendrobium moscatum, has been demonstrated to have excellent anti-cancer activity. The goals of the present study were to investigate the metabolic profiles of moscatilin and to identify and characterize its metabolites. In vitro studies were performed by incubating moscatilin (10 μM) with rat, dog, monkey, and human liver microsomes (0.

DeepMGT-DTI: Transformer network incorporating multilayer graph information for Drug-Target interaction prediction.

Drug-target interaction (DTI) prediction reduces the cost and time of drug development, and plays a vital role in drug discovery. However, most of research does not fully explore the molecular structures of drug compounds in DTI prediction. To this end, we propose a deep learning model to capture the molecular structure information of drug compounds for DTI prediction.

RDFNet: A Fast Caries Detection Method Incorporating Transformer Mechanism.

Dental caries is a prevalent disease of the human oral cavity. Given the lack of research on digital images for caries detection, we construct a caries detection dataset based on the caries images annotated by professional dentists and propose RDFNet, a fast caries detection method for the requirement of detecting caries on portable devices. The method incorporates the transformer mechanism in the backbone network for feature extraction, which improves the accuracy of caries detection and uses the FReLU activation function for activating visual-spatial information to improve the speed of caries detection.

siRNA-Loaded Hydroxyapatite Nanoparticles for Gene Silencing in Anti-Pancreatic Cancer Therapy.

Pancreatic carcinoma (PC) is greatly induced by the gene mutation, but effective targeted delivery for gene therapy has not existed. Small interfering ribonucleic acid (siRNA) serves as an advanced therapeutic modality and holds great promise for cancer treatment. However, the development of a non-toxic and high-efficiency carrier system to accurately deliver siRNA into cells for siRNA-targeted gene silencing is still a prodigious challenge.

Constrained transformer network for ECG signal processing and arrhythmia classification.

Background: Heart disease diagnosis is a challenging task and it is important to explore useful information from the massive amount of electrocardiogram (ECG) records of patients. The high-precision diagnostic identification of ECG can save clinicians and cardiologists considerable time while helping reduce the possibility of misdiagnosis at the same time.Currently, some deep learning-based methods can effectively perform feature selection and classification prediction, reducing the consumption of manpower.

Anti-adipogenic 18,19-seco-ursane stereoisomers and oleane-type saponins from Ilex cornuta leaves.

Three undescribed seco-ursane stereoisomers, ilexcornutosides A-C, two undescribed triterpenoid saponins, ilexcornutosides D-E, and 11 known triterpenoids were isolated from the leaves of Ilex cornuta Lindl. & Paxton. Ilexcornutosides A-C and F with the same planar structures are unique 13(18)-ene-18,19-seco-ursane skeleton triterpenoids, identified as (3S,12R)-3-O-[β-d-glucopyranosyl-(1 → 2)-α-l-arabinopyranosyl]-12-hydroxyl-19-oxo-18,19-secours-13(18)-en-28,21-lactone.

Hydrophobicity and iron coagulation of extracellular polymeric substance from colonial Microcystis.

The bloom-forming cyanobacterium Microcystis occurs mainly as colonial aggregates under the natural conditions. This paper investigated the hydrophobicity and iron coagulation of extracellular polymeric substances (EPSs) from colonial Microcystis in order to understand the impact of EPS on the water treatment process. The higher contents of dissolved EPS (dEPS) and bound EPS (bEPS, mucilaginous matrix around the cells), lower dEPS/bEPS ratio and greater negative zeta potential of bEPS and dEPS were found in colonial Microcystis compared with unicellular Microcystis.

TLC-electrostatic field induced spray ionization-MS analysis of diverse structural skeletons and its coupling with TLC bioautography for characterization of lipase inhibitory components in American ginseng.

Natural compounds with diverse structural skeletons have different ionization efficiencies and different mass spectrometry (MS) responses. Some key factors influencing the electrostatic field induced spray ionization (EFISI)-MS for a variety of natural compounds have been optimized and improved. Fifteen reference substances representing ten well-known skeletons of natural products including alkaloids, flavonoids, phenolic acids, lignans, coumarins, anthraquinones, monoterpenoids, sesquiterpenoids, diterpenoids and triterpenoids, were selected and investigated for their EFISI-MS responses on TLC plates using a dot-blot test.

Aspergilolide, a steroid lactone produced by an endophytic fungus . MBL1612 isolated from .

A new steroid lactone aspergilolide (), and nine known compounds helvolic acid (), verruculogen (), tryprostatin B (), 13-oxofumitremorgin B (), fumitremorgin C (), demethoxy fumitremorgin C (), terezine D (), aszonalenin (), 12, 13-dihydroxy-fumitremorgin C () from cultures of the endophytic fungus . MBL1612. Their chemical structures were determined by a series of extensive spectroscopic methods.

Atypical teratoid rhabdoid tumor mimicking type II neurofibromatosis: A case report.

Rationale: Brain magnetic resonance imaging (MRI) images of atypical teratoid rhabdoid tumor (ATRT) often present heterogeneous signals of various cells without remarkable features of the disease. We describe a unique case of atypical brain MRI images presenting as an type II neurofibromatosis and explore some diagnostic hints.

Patient Concerns: A 1-year-and-7-month-old boy admitted to our department with a 7-day history of drowsiness and 2-day history of emesis, and his presenting complaint was repeated vomit.

Aspergilates A to E, second metabolites from Aspergillus sp. isolated from Paeonia ostii.

Five novel globoscinic acid derivatives, aspergilates A-E (1-5) have been isolated from endophytic fungus Aspergillus sp. derived from Paeonia ostii. The structures of aspergilates A-E were determined by in-depth analyses of NMR spectra, HRESIMS and CD experiments.

[Application of high fibular osteotomy in the treatment of knee osteoarthritis].

Objective: To evaluate the short-and mid-term curative effect of high fibular osteotomy on knee osteoarthritis.

Methods: From October 2014 to July 2016, 76 patients with knee osteoarthritis were treated with high fibular osteotomy. There were 22 males and 54 females, ranging in age from 47 to 82 years old, with an average of 61.

F-fluorodeoxyglucose positron emission computed tomography for monitoring tumor response in esophageal carcinoma treated with concurrent chemoradiotherapy.

The aim of the present study was to explore the value of fluorodeoxyglucose positron emission tomography (F-FDG PET) in monitoring the early tumor response of esophageal squamous cell carcinoma (ESCC) treated with concurrent chemoradiotherapy (CRT). A total of 48 patients with pathologically proven ESCC were retrospectively analyzed. All patients underwent two serial F-FDG PET scans at baseline (pre-CRT) and 40 Gy/4 weeks of starting radiation therapy (inter-CRT).

ErbB3-binding protein 1 inhibits tumor proliferation in esophageal cancer by targeting bcl-2 and p53 gene.

Esophageal cnacer is one of the common malignant tumors with high fatality rate and morbidity, ErbB3-binding protein 1 (EBP1) is the intracellular binding protein of ErbB3. This study aims to investigate the effect of EBP1 on esophageal cancer biological behavior and the underlying mechanism. In our study, esophageal cancer tissues and adjacent non-tumor issues were collected and the relative expression of EBP1 mRNA were examined by real-time PCR.

Cisplatin-based chemoradiotherapy with 5-fluorouracil or pemetrexed in patients with locally advanced, unresectable esophageal squamous cell carcinoma: A retrospective analysis.

Treatment with 5-fluorouracil (5-FU) and cisplatin (PF regimen) remains the most frequently used chemotherapy for esophageal squamous cell carcinoma (SCC). The aim of the present study was to assess the efficacy and safety of pemetrexed/cisplatin (PP regimen) as definitive treatment compared with PF. A total of 60 patients with locally advanced, unresectable SCC of the esophagus receiving concomitant chemoradiotherapy were recruited in this study; of those patients, 29 received four cycles (two concomitant and two post-radiotherapy) of the PF regimen (arm A, cisplatin 25 mg/m/day i.

Severe Chromoblastomycosis-Like Cutaneous Infection Caused by .

species are saprophytic filamentous fungi commonly found in the soil, dung, and animal fur. Subcutaneous infection caused by this organism is rare in humans. We report a case of subcutaneous fungal infection caused by in a 38-year-old woman.

Synovial Fluid Macrophage Migration Inhibitory Factor Levels Correlate with Severity of Self-Reported Pain in Knee Osteoarthritis Patients.

BACKGROUND Inflammation is considered as one of the main pathogeneses in OA-induced pain. Macrophage migration inhibitory factor (MIF) is a well known pro-inflammatory cytokine. We aimed to determine whether MIF levels in serum and synovial fluid (SF) are associated with severity of OA-induced pain.

Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma.

MicroRNA-7 has been reported to participate in tumorigenesis and progression by several signaling pathways in various tumors. However, its potential as a serum diagnostic factor and predictive biomarker for esophageal squamous cell carcinoma (ESCC) has not been studied. Serum samples were collected from 105 pathologically proven ESCC patients and 30 age- and gender-matched healthy controls.