Chlorella's transport inhibition: A powerful defense against high ammonium stress.

Ammonium (NH₄⁺) is a primary nitrogen source for many species, yet NH₄⁺-rich wastewater presents a substantial risk to environment. Chlorella sorokiniana is widely recognized for wastewater treatment. The development of high NH₄⁺ tolerant strains has the potential to significantly enhance wastewater treatment efficiency and reduce treatment costs.

Mechanistic insights for efficient removal of intracellular and extracellular antibiotic resistance genes by iron-based nanocopper: Intracellular oxidative stress and internalization of nanocopper.

The widespread use of antibiotics has led to a severe pollution issue with antibiotic resistance genes (ARGs), which poses a significant threat to both ecological environments and human health. In this study, we developed an iron-based nanocopper bimetallic material (Fe-nCu) for the efficient removal of ARGs. Our results indicate that nCu can attach to the surface of iron, forming aggregated copper nanoclusters resembling wheat ears.

Distribution and driving mechanisms of antibiotic resistance genes in urbanized watersheds.

Antibiotic resistance genes (ARGs) have emerged as a global concern, posing significant threats to human health and safety. Understanding the contamination levels and driving mechanisms behind ARG proliferation is urgently needed. Urban watersheds, influenced by human activities, serve as critical reservoirs for ARGs; however, the impact of urbanization on ARG spread of and the underlying driving mechanisms remain unclear.

Ammonia oxidizing bacteria (AOB) denitrification and bacterial denitrification as the main culprit of high NO emission in SBR with low C/N ratio wastewater.

A large amount of greenhouse gas nitrous oxide (NO) will be produced during the biological nitrogen removal process for organic wastewater of low C/N ratio. One of the effective methods to solve this problem is to incorporate inexpensive carbon source. In this study, raw wastewater (RW) from pig farm, that was not anaerobically digested, was utilized as exogenous carbon in both A/O and SBR aerobic reactor to treat liquid digestate with high ammonia nitrogen and low C/N ratio.

DROEG: a method for cancer drug response prediction based on omics and essential genes integration.

Predicting therapeutic responses in cancer patients is a major challenge in the field of precision medicine due to high inter- and intra-tumor heterogeneity. Most drug response models need to be improved in terms of accuracy, and there is limited research to assess therapeutic responses of particular tumor types. Here, we developed a novel method DROEG (Drug Response based on Omics and Essential Genes) for prediction of drug response in tumor cell lines by integrating genomic, transcriptomic and methylomic data along with CRISPR essential genes, and revealed that the incorporation of tumor proliferation essential genes can improve drug sensitivity prediction.

-Acyl-Homoserine Lactone (AHL)-Mediated Microalgal-Bacterial Communication Driving Activated Sludge Bacterial Biofloc Formation.

-acyl-homoserine lactones (AHLs) as autoinducers of Gram-negative bacteria for quorum sensing regulation have shown positive effects on the production of aromatic proteins in extracellular polymeric substances (EPSs) during bioflocculation. To investigate the role of AHLs in aromatic protein production, a -bacteria system with great bioflocculation was established via fed-batch cultivation. Tryptophan and aromatic proteins as the main compounds in the EPS of bioflocs showed an increasing trend during fed-batch cultivation.

Nitrification performance evaluation of activated sludge under high potassium ion stress during high-ammonia nitrogen organic wastewater treatment.

The recycling reverse osmosis (RO) membrane concentrate of some high-ammonia nitrogen (NH-N) organic wastewater to the biological unit could cause potassium ion (K) accumulation, thereby affecting the removal of NH-N by activated sludge. Thus, the effects of high K stress on activated sludge nitrification performance was studied. The results showed that the high K stress promoted the floc sludge to produce more extracellular polymers (EPS), which accelerated the sludge sedimentation and enriched the biomass in sequential batch reactors (SBRs).