Hematin and Cu control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion.

We present a significant finding that Cu(II) ions can activate hematin (Hem) to generate more HOO⋅ and O radicals from the decomposition of HO. Galactose oxidase (GO) and hematin have been simultaneously immobilized by coordinating to Cu(II) ions (GO&Hem@Cu(II)). The radicals HOO⋅ and O and dioxygen O can be generated from the byproduct HO by the Cu(II)-activated hematin.

Cobalt Doped in Zn-MOF-5 Nanoparticles to Regulate Tumor Microenvironment for Tumor Chemo/Chemodynamic Therapy.

Metal-organic frameworks are often used as a chemotherapeutic drug carrier due to their diverse metal sites and good acid degradation ability. Herein Co-doped Zn-MOF-5 nanoparticles with a high Co doping rate of 60% were synthesized for chemo-chemodynamic synergistic therapy of tumor. Co ions can mediate chemodynamic therapy through Fenton-like reaction and regulate the tumor microenvironment by consuming the reduced glutathione.

Effects of BMI1 Gene on Regulating Apoptosis, Invasion, and Migration of HEC-1B Cells Induced by Ionizing Radiation.

The aim of this study was to examine the role of B lymphoma Moloney murine leukemia virus insertion region 1 (BMI1) gene in regulating the apoptosis, invasion, and migration of human endometrial adenocarcinoma cell line (HEC-1B) cells induced by ionizing radiation. The expression of BMI1 mRNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the positive expression of BMI1 was detected by immunohistochemistry (IHC) staining. HEC-1 B cells were randomly divided into three groups: control group, BMI1 overexpression group, and BMI1 inhibitor group.

Fabrication of a Fibrous Metal-Organic Framework and Simultaneous Immobilization of Enzymes.

A nanorod-like lanthanum metal-organic framework (LaMOF) was synthesized in aqueous solution by coordinating La(III) to the ligand 1,3,5-benzenetricarboxylic acid. The fibrous LaMOF was fabricated by splitting the nanorod-like LaMOF in a solution of d-amino acid oxidase, and the enzyme was immobilized simultaneously. Based on SEM and TEM images, STEM mapping, and spectra of XPS and FTIR, the mechanism of formation of the fibrous LaMOF and the distinct interfacial phenomena have been elucidated.

Heparin Immobilized on Multiwalled Carbon Nanotubes for Catalytic Conversion of Fructose in Water with High Yield and Selectivity.

Being a member of the glycosaminoglycan family of carbohydrates, native heparin is a highly sulfated polysaccharide. Herein, heparin was grafted onto polydopamine (PDA)- and poly(ethylene imine) (PEI)-coated multiwalled carbon nanotubes (MWCNTs) (heparin-PEI@PDA@MWCNT). The immobilized heparin consists of a sulfated repeating disaccharide unit, conferring a unique microenvironment when catalyzing fructose dehydration into 5-hydroxymethylfurfural (HMF).

Immobilization of R-ω-transaminase on MnO nanorods for catalyzing the conversion of (R)-1-phenylethylamine.

R-ɷ-transaminases transfer an amino group from an amino donor (e.g. (R)-1-phenylethylamine) onto an amino acceptor (e.

A two-enzyme immobilization approach using carbon nanotubes/silica as support.

Multiple enzyme mixtures are attractive for the production of many compounds at an industrial level. We report a practical and novel approach for coimmobilization of two enzymes. The system consists of a silica microsphere core coated with two layers of individually immobilized enzymes.

Sodium hexadecyl sulfate as an interfacial substance adjusting the adsorption of a protein on carbon nanotubes.

Carbon nanotubes (CNTs) were functionalized with sodium hexadecyl sulfate (SHS). The lysozyme adsorbed on the SHS-CNTs exhibited a higher activity than that immobilized on the nonfunctionalized CNTs. To explain the experimental results and explore the mechanism of lysozyme adsorption, large-scale molecular dynamics simulations have been performed for a four-component system, including lysozyme, SHS, CNTs in explicit water.

Molecular mechanism of the interactions between inhibitory tripeptides and angiotensin-converting enzyme.

Angiotensin I-converting enzyme (ACE) is a key therapeutic target for combating hypertension and related cardiovascular diseases. ACE inhibitory peptides offer the prospect of enhanced potency, high specificity, and no or low side effect. The ACE inhibitory tripeptides LKP and IKP differ from each other by one amino acid but their inhibitory potencies for ACE differ significantly.

Synthesis of bioethanol from biomass-derived syngas over carbon nanotube/silica supported catalyst.

Multi-walled carbon nanotubes (MWNTs) were functionalized with pyrogallol and used in a composite with silica as a support for a Cu-Co based catalyst. The catalysts were characterized using X-ray diffraction, transmission electron microscopy, and H(2) temperature programmed reduction. The effects of pyrogallol and the weight ratio of silica to MWNTs on the performance of the catalyst were studied in a fixed bed reactor.

Lipase immobilized on magnetic multi-walled carbon nanotubes.

Magnetic iron oxide nanoparticles were loaded onto the surfaces of multiwalled carbon nanotubes (MWNTs) by the impregnation method. The obtained magnetic nanotubes were characterized with high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD). Yarrowia lipolytica lipase was covalently immobilized on the magnetic MWNTs (M-MWNTs).

Enzymes immobilized on carbon nanotubes.

Enzyme immobilizations on carbon nanotubes for fabrication of biosensors and biofuel cells and for preparation of biocatalysts are rapidly emerging as new research areas. Various immobilization methods have been developed, and in particular, specific attachment of enzymes on carbon nanotubes has been an important focus of attention. The method of immobilization has an effect on the preservation of the enzyme structure and retention of the native biological function of the enzyme.