Publications by authors named "Peihang Fang"
Estrogen and BRCA1 deficiency synergistically induce breast cancer mutation-related DNA damage.
Biochem Biophys Res Commun
July 2022
Estrogen (E2) is crucial for the development of breast cancer caused by BRCA1 mutation, and can increase the DNA damage in BRCA1-deficient cells. However, the mechanisms through which BRCA1 deficiency and E2 synergistically induce DNA damage remains unclear. In this study, we analyzed the distribution of DNA damage in E2-treated BRCA1-deficient cells.
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- A protocol is introduced to manipulate the phase separation capacity of proteins, specifically focusing on mutations in intrinsically disordered regions (IDRs).
- By mutating specific residues, the study can disrupt phase separation, which is crucial for cell fate transitions.
- The researchers also explore ways to restore phase separation by fusing IDRs known to drive it, highlighting the challenge of predicting which mutations will be effective.
Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions.
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