Nondestructive detection of saline-alkali stress in wheat (Triticum aestivum L.) seedlings via fusion technology.

Background: Wheat (Triticum aestivum L.) is an important grain crops in the world, and its growth and development in different stages is seriously affected by saline-alkali stress, especially in seedling stage. Therefore, nondestructive detection of wheat seedlings under saline-alkali stress can provide more comprehensive technical support for wheat breeding, cultivation and management.

Flexible and wearable sensor for in situ monitoring of gallic acid in plant leaves.

Gallic acid (GA) is one of the main phenolic components naturally occurring in many plants and foods and has been a subject of increasing interest owing to its antioxidant and anti-mutagenic properties. This study introduces a novel flexible sensor designed for in situ detecting GA in plant leaves. The sensor employs a laser-induced graphene (LIG) flexible electrode, enhanced with MXene and molybdenum disulfide (MoS) nanosheets.

Genome-wide analysis of MYB transcription factor family and AsMYB1R subfamily contribution to ROS homeostasis regulation in Avena sativa under PEG-induced drought stress.

Background: The myeloblastosis (MYB) transcription factor (TF) family is one of the largest and most important TF families in plants, playing an important role in a life cycle and abiotic stress.

Results: In this study, 268 Avena sativa MYB (AsMYB) TFs from Avena sativa were identified and named according to their order of location on the chromosomes, respectively. Phylogenetic analysis of the AsMYB and Arabidopsis MYB proteins were performed to determine their homology, the AsMYB1R proteins were classified into 5 subgroups, and the AsMYB2R proteins were classified into 34 subgroups.

Development of surface molecular-imprinted electrochemical sensor for palmitic acid with machine learning assistance.

Palmitic acid (PA) is a kind of saturated high fatty acid, which is involved in physiological safety and food quality. A surface molecularly imprinted polymer (MIP) electrochemical sensor was prepared on MXene surface using dopamine (DA) as functional monomer. The electrode was modified with gold nanoparticles (AuNPs), ferrocene-graphene oxide-multiwalled carbon nanotubes (Fc-GO-MWCNT) composite to enhance the electroactive area and conductivity.

An ultrasensitive probe-free electrochemical immunosensor for gibberellins employing polydopamine-antibody nanoparticles modified electrode.

Gibberellins (GA) is an ubiquitous plant hormone, which plays a regulatory role in different growth stages of plants, so it is of great significance to develop a sensitive quantitative analysis method for GA. In this study, carboxylated graphene oxide- carboxylated multi-walled carbon nanotubes-Fc (GO-MWNT-Fc) composite material and PDANPs-antibody (PDANPs-Ab) were sequentially modified to screen-printed electrodes (SPEs), and an ultrasensitive probe-free immunosensor for GA was developed. Fc was applied to generate electrochemical signals.

A novel COOH-GO-COOH-MWNT/pDA/AuNPs based electrochemical aptasensor for detection of AFB.

Aflatoxin B (AFB), one of the most common mycotoxins in food matrixes, has been identified as the most toxic contaminant with mutagenic, teratogenic, immunosuppressive, and carcinogenic effects. In this study, an electrochemical aptamer sensor was developed for the on-site detection of AFB. Carboxylated graphene oxide (COOH-GO) and carboxylated multi-walled carbon nanotubes (COOH-MWNT) nanocomposites, dopamine polymers (pDA) and gold nanoparticles (AuNPs) were used to enhance the electrochemical activity and the biocompatibility of the screen-printed electrodes (SPE).

In Vivo Detection of Glutamate in Tomatoes by an Enzyme-Based Electrochemical Biosensor.

The in vivo and on-site detection of key physiology parameters in plants will be of great relevance for precision agriculture and food technology. In this work, a sensitive enzymatic glutamate sensor was successfully developed. To enhance the conductivity and catalytic ability and to fix the glutamate oxidase, Au-Pt nanoparticles were first deposited on screen-printed electrodes, and then carboxylated graphene oxide and carboxylated multiwalled carbon nanotubes were fabricated for the synthesis of the electrode.

Enzyme-Free Electrochemical Sensors for Quantification of Reducing Sugars Based on Carboxylated Graphene-Carboxylated Multiwalled Carbon Nanotubes-Gold Nanoparticle-Modified Electrode.

The reducing sugars of plants, including glucose, fructose, arabinose, galactose, xylose, and mannose, are not only the energy source of plants, but also have the messenger function of hormones in signal transduction. Moreover, they also determine the quality and flavor of agricultural products. Therefore, the quantification of reducing sugars in plants or agriculture products is very important in precision agriculture.

A probe-free electrochemical immunosensor for methyl jasmonate based on ferrocene functionalized-carboxylated graphene-multi-walled carbon nanotube nanocomposites.

Methyl jasmonate (MeJA) is an endogenous plant hormone, which plays an important role in agriculture production. A novel probe-free electrochemical immunosensor was fabricated for detecting of MeJA. Fc, carboxylated graphene (COOH-GR) and carboxylated multi-walled carbon nanotubes (COOH-MWNT) composite was formed and used to fabricate screen-printed electrode (SPE).

Antioxidant Enzymatic Activity and Osmotic Adjustment as Components of the Drought Tolerance Mechanism in .

Drought stress is a major environmental constraint for plant growth. Climate-change-driven increases in ambient temperatures resulted in reduced or unevenly distributed rainfalls, leading to increased soil drought. C.

Thioredoxin f Confers Osmotic Stress Tolerance in Transgenic Tobacco.

Water deficit caused by osmotic stress and drought limits crop yield and tree growth worldwide. Screening and identifying candidate genes from stress-resistant species are a genetic engineering strategy to increase drought resistance. In this study, an increased concentration of mannitol resulted in elevated expression of thioredoxin f () in the nonsecretor mangrove species .

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid.

Indole-3-acetic acid (IAA) and salicylic acid (SA) are two important phytohormones. In this work, for the first time, a ratiometric electrochemical sensor was developed for quantifying IAA and SA simultaneously. A composite of multi wall carbon nanotubes (MWNT) and carbon black (CB) was used to enhance the sensitivity of electrochemical detection.

Illumination/Darkness-Induced Changes in Leaf Surface Potential Linked With Kinetics of Ion Fluxes.

A highly reproducible plant electrical signal-light-induced bioelectrogenesis (LIB) was obtained by means of periodic illumination/darkness stimulation of broad bean ( L.) leaves. By stimulating the same position of the same leaf with different concentrations of NaCl, we observed that the amplitude and waveform of the LIB was correlated with the intensity of stimulation.

Disposable stainless steel-based electrochemical microsensor for in vivo determination of indole-3-acetic acid in soybean seedlings.

In vivo detecting of plants signal molecules is of great importance for the precision farming, crop management and plant phenotyping. In this work, for in vivo detecting indole-3-acetic acid (IAA), one of phytohormones, fine stainless steel (SS) wire was used as electrode material. Highly ordered nanopores, popcorn-like Au nanostructures, Pt nanoparticles and reduced graphene oxide (ERGO) nanocomposite films, and polymerized ST film (PST) were fabricated on the SS microelectrode in turn for improving the detection effect.

detection of salicylic acid in sunflower seedlings under salt stress.

Salicylic acid (SA) is an important phytohormone. It plays an essential role in regulating many physiological processes of plants. Most of the conventional methods for SA detection are based on processes.

Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants.

Apyrase and extracellular ATP play crucial roles in mediating plant growth and defense responses. In the cold-tolerant poplar, Populus euphratica, low temperatures up-regulate APYRASE2 (PeAPY2) expression in callus cells. We investigated the biochemical characteristics of PeAPY2 and its role in cold tolerance.

Populus euphratica HSF binds the promoter of WRKY1 to enhance salt tolerance.

Poplar species increase expressions of transcription factors to deal with salt environments. We assessed the salt-induced transcriptional responses of heat-shock transcription factor (HSF) and WRKY1 in Populus euphratica, and their roles in salt tolerance. High NaCl (200mM) induced PeHSF and PeWRKY1 expressions in P.

Overexpression of copper/zinc superoxide dismutase from mangrove Kandelia candel in tobacco enhances salinity tolerance by the reduction of reactive oxygen species in chloroplast.

Na(+) uptake and transport in Kandelia candel and antioxidative defense were investigated under rising NaCl stress from 100 to 300 mM. Salinized K. candel roots had a net Na(+) efflux with a declined flux rate during an extended NaCl exposure.

Overexpression of PeHA1 enhances hydrogen peroxide signaling in salt-stressed Arabidopsis.

The plant plasma membrane (PM) H(+)-ATPase plays a crucial role in controlling K(+)/Na(+) homeostasis under salt stress. Our previous microarray analysis indicated that Populus euphratica retained a higher abundance of PM H(+)-ATPase transcript versus a salt-sensitive poplar. To clarify the roles of the PM H(+)-ATPase in salt sensing and adaptation, we isolated the PM H(+)-ATPase gene PeHA1 from P.

Exogenous hydrogen peroxide, nitric oxide and calcium mediate root ion fluxes in two non-secretor mangrove species subjected to NaCl stress.

Using 3-month-old seedlings of Bruguiera gymnorrhiza (L.) Savigny and Kandelia candel (L.) Druce, we compared species differences in ionic homeostasis control between the two non-secretor mangrove species.

Different modulating effects of adenosine on neonatal and adult polymorphonuclear leukocytes.

Polymorphonuclear leukocytes (PMNs) are the major leukocytes in the circulation and play an important role in host defense. Intact PMN functions include adhesion, migration, phagocytosis, and reactive oxygen species (ROS) release. It has been known for a long time that adenosine can function as a modulator of adult PMN functions.