[Construction of MHV-A59 damp-heat mouse model and analysis of the relevant indices].

Objective: To explore the impact of inflammation, water metabolism and immune function on the establishment of a mouse model of damp-heat syndrome with MHV-A59 infection.

Methods: Twenty-four mice were randomly divided into control group, virus group, damp-heat group and model group. The peripheral blood CD4(+) and CD8(+) lymphocytes were detected by flow cytometry, and the serum levels of IFN-γ and IL-4 were assayed by ELISA.

[The mechanism of signal extension in Haoqin Qingdan decoction immunity activity in Damp-heat syndrome of pneumonia disease infected by influenza virus].

Objective: To explore the immunoregulation existing signal transduction mechanism, to evaluate the role of lay its experimental basis By using Haoqin Qingdan decoction for treatments on the mouse models.

Methods: A total of 40 NIH Mice were randomly divided into five groups: control group, virus group (infecting by influenza virus), complex model group (richly fatty and sweet diet + Humid heat environment + infecting by influenza virus), virazole group (mouse of model group was treated by virazole), and Haoqin Qingdan decoction group (mouse of complex model group was treated by decoction of Haoqin Qingdan). When the complex model was established, determination of the mice lung indexes in each group and calculate the inhibition of lung indexes.

[Effect of anti-influenza virus H3N2 of Hypericum japonicum in vivo].

Objective: To investigate anti-influenza virus H3N2 effect of Hypericum japonicum in vivo.

Methods: The influences on lung index and death rate were observed in the mice infected with virus H3N2 from intranasal.

Results: Experiments in vivo showed that Hypericum japonicum at the concentration of 10 g/kg x d for the intranasal treatment markedly inhibited the lung consolidation of mice pneumonia caused by the infection of influenza virus H3N2 and prolonged the survival time .

[Clinical trial and evaluation on comprehensive treatment on attack in acute stage: report of 522 cases].

Objective: To investigate the clinical effect of the comprehensive treatment to acute stage of attack.

Methods: On the basis of the previous observation, the study of the randomization control with general treatment, treatment on acupuncture and western medical treatment were carried out. A comprehensive treatment on overall traditional Chinese medical differentiation according to the superiority of every treatment was assessed and evaluated in 522 patients with attack.