[Tension-force induced cyclooxygenase-2 expression mediated by microfilament in human periodontal ligament fibroblast].

Objective: To study the role of microfilament polymerization in menchanotransduction by human periodontal ligament fibroblast (hPDLFs).

Methods: In tension-force group, hPDLFs were treated by tension-force values of 18% for 8 h, 16 h, 24 h. In tension-force and inhibitor group, the sample was treated with 5 microg/mL cytochalasin B before using tension-forece.

[Effect of continuously compressive pressure on the expression of RANKL mRNA in human periodontal ligament cells in vitro].

Objective: To determine the effect of continuously compressive pressure (CCP) on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) in human periodontal ligament cells (HPDLCs) and to investigate the role of RANKL in alveolar bone rebuilding during orthodontic tooth movement.

Methods: The primary HPDLCs were isolated from human periodontal ligament by explanting enzymatic digestion with trypsin and collagenase to establish a pressure model. Top-bottom axial pressures (1, 2, and 3 g/cm(2)) were laid on HPDLCs for 0.