Gene expression profiling and pathway network analysis of anti-tumor activity by Jaridon 6 in esophageal cancer.

Jaridon 6, a novel ent-kaurene diterpenoid derived from Rabdosia rubescens (Hemsl.) Hara, possesses strong anti-tumor activity in esophageal cancer cells. In this study, we explored the underlying molecular events of the anti-tumor activity of Jaridon 6.

[Apoptosis and growth arrest of human esophageal squamous cell carcinoma cell EC9706 induced by Fufangkushen injection].

Objective: To investigate the inhibitory effect and apoptosis induction on human esophageal carcinoma EC9706 cell by Fufangkushen.

Methods: The experiment of Fufangkushen was designed into three groups including 25.00 µl/ml group, 6.

[Up-regulation of stathmin induces growth arrest of esophageal squamous cell carcinoma EC9706 cell].

Objective: To construct a eukaryotic expression vector of stathmin gene and investigate its effect on esophageal squamous cell carcinoma (ESCC) EC9706 cell line.

Methods: Stathmin cDNA coding sequence was amplified by RT-PCR and cloned into a eukaryotic expression vector pcDNA3.1(+).

[Expression of stathmin in esophageal squamous cell carcinoma and its biological significance].

Objective: To explore the expression of stathmin gene in esophageal squamous cell carcinoma (ESCC) and its correlation to oncogenesis of ESCC.

Methods: Three ESCC cell lines, 75 ESCC samples, 25 tumor-adjacent samples and 30 normal esophageal mucosa samples were examined for the expression of stathmin mRNA and protein by in situ hybridization and immunohistochemistry, respectively. The correlations of stathmin expression to the clinicopathological features of the patients were analyzed.

[Construction and expression of human stathmin gene eukaryotic expression vector and its effect on esophageal cancer cells].

Objective: To construct an eukaryotic expression vector of human stathmin gene and to assess its effect on esophageal cancer EC9706 cells.

Methods: Stathmin cDNA coding sequence was amplified by RT-PCR from Eca109 cells and was cloned into pMD18-T vector. After identifying and sequencing, the correct inserting stathmin gene was sub-cloned into eukaryotic expression vector pEGFP-C2.

[Apoptosis in esophageal cancer cells induced by all-trans retinoic acid].

Objective: To study the anti-tumor effects of all-trans retinoic acid (ATRA) and mechanisms of its action.

Methods: Human esophageal carcinoma cell line EC9706 cells were treated with ATRA at different concentration. The proliferation inhibition was examined by MTT assay.

[Demethylation of estrogen receptor gene and its re-expression in estrogen receptor-negative breast].

Objective: To investigate the correlation between the lack of estrogen receptor (ER) gene expression and hypermethylation of ER gene, and detect whether re-expressed ER protein is activated.

Methods: The methylation status of ER gene promoter in the ER-negative breast cancer cells was evaluated by methylation specific PCR (MSP) and genomic sequencing. The expression of ER and progesterone receptor (PR) mRNA as well as the production of ER protein were detected by RT-PCR and Western blot method, respectively.