[The tk gene mutation analysis in WTK1 cell line by multiplex PCR].

Objective: To explore the tk gene mutation spectrum in spontaneous and induced WTK1 mutants.

Methods: After exposure to methyl methanesulfonate (MMS), mitomycin (MMC) or sodiam azide (NaN3), spontaneously-arising and induced mutants of WTK1 cell line were selected. The spectrum and hotspot of tk gene mutation were analyzed by multiplex PCR.

[Comparison of TK gene mutation assay in TK6 and TK6-E6 cell induced by vinblastin and colcemid].

Objective: To compare TK gene mutation assay in human lymphoblastoid cell lines TK6 and TK6-E6 induced by vinblastin and colcemid.

Methods: Regular TK gene mutation assays were experimented to detect cytotoxicity and mutation frequency at tk locus after TK6 and TK6-E6 human lymphoblastoid cells were treated with vinblastin and colcemid for 24h.

Results: Relative survival (RS%) and Relative suspension growth (RSG%) of TK6 and TK6-E6 cells decreased when concentrations of vinblastin and colcemid increased.

[Analysis of loss of heterozygosity induced by vinblastine at tk locus in human lymphoblastoid cell line TK6].

Objective: To establish TK gene mutation assay using human lymphoblastoid cell line TK6 and to study the genotoxic mechanism of Vinblastine (VBL).

Methods: TK6 cells were treated with Vinblastine at different concentrations (0.625 ng/mL, 1.

[TK locus mutation assay: comparison of L5178Y and TK6 cell lines].

Objective: To compare the advantages and disadvantages of utilizing L5178Y and TK6 cell lines in the TK locus mutation assay.

Methods: The two cell lines were used for detecting and assessing the mutability of four chemicals (MMS, EMS, MMC and KCl); the microwell method of TK locus mutation assay was adopted.

Results: The two cell lines brought about similar results in the study.