[Construction of eukaryotic expression recombinant plasmid of trichomonas vaginalis ferredoxin gene].

Total DNA was extracted from T. vaginalis with Chelex-100 method and used as templates for PCR. The ferredoxin gene was directionally cloned into plasmid pMD-18T vector and subcloned into eukaryotic expression vector pcDNA3.

[Cloning and sequence analysis of Trichomonas vaginalis ferredoxin gene].

Objective: To construct a recombinant plasmid containing ferredoxin gene of Trichomonas vaginalis.

Methods: Total DNA was extracted from Trichomonas vaginalis with Chelex-100 method and used as templates for PCR. Primers were designed based on the published sequence of the ferredoxin gene and used to amplify the Trichomonas vaginalis gene using PCR method.