Blockade of L-type calcium channel in myocardium and calcium-induced contractions of vascular smooth muscle by CPU 86017.

Aim: To assess the blockade by CPU 86017 on the L-type calcium channels in the myocardium and on the Ca(2+)-related contractions of vascular smooth muscle.

Methods: The whole-cell patch-clamp was applied to investigate the blocking effect of CPU 86017 on the L-type calcium current in isolated guinea pig myocytes and contractions by KCl or phenylephrine (Phe) of the isolated rat tail arteries were measured.

Results: Suppression of the L-type current of the isolated myocytes by CPU 86017 was moderate, in time- and concentration-dependent manner and with no influence on the activation and inactivation curves.

Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons.

Aim: To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice.

Methods: The whole-cell patch clamp technique was used.

Results: Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L.

Puerarin blocks Na+ current in rat ventricular myocytes.

Aim: To study the effect of puerarin (Pue) on Na+ channel in rat ventricular myocytes.

Methods: Whole-cell patch-clamp technique was applied on isolated cardiomyocytes from rats.

Results: Pue inhibited cardiac INa in a positive rate-dependent and dose-dependent manner, with an IC(50) of 349 micromol/L.