[Mechanism of heat shock protein 90 for regulating 26S proteasome in hyperthermia].

Objective: To investigate the mechanism by which heat shock protein 90 (HSP90) regulates 26S proteasome in hyperthermia.

Methods: Hyperthermic HepG2 cell models established by exposure of the cells to 42 degrees celsius; for 3, 6, 12, and 24 h were examined for production of reactive oxygen species (ROS) and cell proliferation, and the changes in Hsp90α and 26S proteasome were analyzed.

Results: ROS production in the cells increased significantly after hyperthermia (F=28.

[Synthesis and Characterization of PAM Derivative Weak Gel].

Weak gel with PAM cross-linked radical reaction was prepared by being incorporated to side chain of PAM. The structure, gel for ming process and the influence of NaCl concentration to apparent viscosity of gel were studied. The results show that the crosslinking ratios influence to crosslinking time, gel breaking time and gel strength.