Development of an Escherichia coli whole cell biocatalyst for the production of hyperoside.

Objective: To produce high concentrations of hyperoside from quercetin using recombinant Escherichia coli with in situ regeneration of UDP-galactose.

Results: Sucrose synthase from Glycine max (GmSUS) was co-expressed with UDP-glucose epimerase from E. coli (GalE) in E.

Engineering a heterologous synthetic pathway in Escherichia coli for efficient production of biotin.

Objective: To enhance biotin production in Escherichia coli by engineering a heterologous biotin synthetic pathway.

Results: Biotin operon genes from Pseudomonas putida, which consisted of a bioBFHCD cluster and a bioA gene, was engineered into Escherichia coli for biotin production. The introduction of bioW gene from Bacillus subtilis, encoding pimeloyl-CoA synthetase and sam2 gene from Saccharomyces cerevisiae, encoding S-adenosyl-L-methionine (SAM) synthetase contributed to the heterologous production of biotin in recombinant E.