Effect of Y-27632 on the cultured retinal neurocytes of rats.

Aim: To investigate the effect of Y-27632 on the survival and neurite outgrowth of the cultured retinal neurocytes.

Methods: After the postnatal day 2-3, Sprague-Dawley retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum-free media (control group) and serum-free media contained 30µmol/L Y-27632 (Y-27632 group), and the cells were continually cultured another 48 hours. The cultured retinal neurocytes were identified with anti-neuron specific enolase (NSE) immunocytochemistry.