[The influence of different alkaline pH conditions on Enterococcus faecalis in planktonic and biofilm mode].

Purpose: To study the influence of different pH conditions on Enterococcus faecalis(E. faecalis) in planktonic and biofilm mode.

Methods: E.

Low-temperature heat capacities of 1-alkyl-3-methylimidazolium bis(oxalato)borate ionic liquids and the influence of anion structural characteristics on thermodynamic properties.

Two chelated orthoborate ionic liquids (ILs), 1-butyl-3-methylimidazolium bis(oxalato)borate ([Bmim][BOB]) and 1-hexyl-3-methylimidazolium bis(oxalato)borate ([Hmim][BOB]), were prepared and characterized. Their thermodynamic properties were studied using adiabatic calorimetry and differential scanning calorimetry (DSC). The thermodynamic properties of the two ILs were evaluated and compared with each other, and then with those of other [Bmim] type ILs.

[Quantitative evaluation of residual endodontic microorganisms after mechanical root canal preparation different chemical preparations by real-time PCR].

Purpose: To establish a quick, sensitive method for quantifying root canal flora and investigate the effects of different root canal preparations on the pathogenic bacteria at RNA level.

Methods: A total of 24 single-rooted teeth with chronic apical periodontitis were selected and prepared using 3% H2O2 combined with 1% NaClO, EDTA combined with 3% H(2)O(2),1% NaClO, respectively,the samples were taken before and after root canal preparation. After isolation of total RNA from the root canal samples, cDNA was synthesized by reverse transcription, and detected by real-time PCR.

[Quantitive study of interleukin 1beta in periapical exudates of chronic periapical periodontitis in the course of root canal therapy].

Purpose: To evaluate the relationship between IL-1beta and clinical findings of chronic apical periodontitis and to explore the function of IL-1beta during the endodontic interappointment flare-ups.

Methods: Periapical exudates samples were obtained from 19 teeth suffering from endodontic flare-ups after root canal preparation and 20 teeth without any symptoms and signs at the second visit after root canal preparation. The levels of IL-1beta were determined by ELISA and the data was analyzed by SAS6.