[Effectiveness evaluation of dynamic automatic identification system (device) of miracidia].

Objective: To develop a dynamic automatic identification system (device) of miracidia to achieve the automatic detection and improve the detection rate and efficiency of schistosome miracidia.

Methods: The dynamic automatic identification system (device) of miracidia was composed of an optical stereoscope, a digital camera, dynamic automatic tracking recognition software, and a computer. Under different conditions, the detection rates and efficiency of the system were compared with those of five professional persons.

[ infection in Nanjing City from 2015 to 2016].

Objective: To investigate the status of infection in the population in Nanjing City so as to provide the evidence for the prevention and control of cryptosporidiosis.

Methods: A total of 100 fecal samples were collected from each of three districts (Liuhe, Qixia and Gaochun) and one hospital (Nanjing Zhongda Hospital) in 2015 and 2016 respectively. The fecal samples were detected for with microscopy (by using the gold amine phenol-modified acid-fast staining) and the positive samples were detected again for the molecular biology confirming by using the fluorescence quantitative PCR.

[Comparison of development between single sexual infection and double sexual infection in mice and rabbits].

Objective: To observe the development of after single sexual infection and double sexual infection respectively.

Methods: A single snail was infected by a single schistosome miracidium. The larva were induced and released from the snail after 60-day incubation at 26 ℃.

[Impact of implementation of Three Gorges Project on schistosomiasis endemic situation and control in Nanjing City].

Objective: To understand the changes of hydrological regime in the Yangtze River and schistosomiasis endemic situation in Nanjing City after the implementation of Three Gorges Project.

Methods: The data of hydrological regime of the Yangtze River, schistosomiasis epidemic situation, and Oncomelania hupensis snail status were collected and analyzed in Nanjing City from 2002 to 2012.

Results: After the implementation of the Three Gorges Project in 2003, the water level of the Yangtze River slightly rose from January to April, the average water levels in May and August both reduced compared with those in 2002, and the time of water withdrawal was moved up.

[Imported falciparum malaria infections in Nanjing City from 2008 to 2012].

Objective: To understand the situation of imported falciparum malaria infections in Nanjing City so as to provide the evidence for formulating the control strategy of malaria.

Methods: The data of imported falciparum malaria cases in Nanjing City were collected and analyzed.

Results: From 2008 to 2012, the imported falciparum malaria cases reported were 3, 5, 15, 17, 18, respectively, and the total cases were 58, with the increasing trend year by year.

[Automatic microscopic scanning system in etiological diagnosis of schistosomiasis].

Objective: To apply the automatically microscopic scanning system in the etiological diagnosis of schistosomiasis, as an alternative to the traditional manual microscopy detection, and improve the detection rates and efficiency of examinations of fecal slides.

Methods: A single-blind method was used throughout the process of both the automatic microscopic scanning system and traditional manual microscopy reading. Each of five professionals read 20 Kato-Katz manure piece samples.

[Improvement of fully automatically microscopic scanning system based on the oil-lens and its application in reading of malaria blood smears].

Objective: To improve the automatically microscopic scanning system based on the oil-lens and apply it in reading of malaria blood smears.

Methods: The software of the system was modified and optimized. A total of 115 blood films from Tengchong City of Yunnan Province were examined by the automatically systematic method or read by blood smear examination staff of Tengchong City and the accuracy, sensitivity and specificity were determined and compared between the two methods.