TRP36-ELISA for detection: Concordance with TaqMan real-time PCR and point-of-care testing.

, a widely distributed tick-borne pathogen, requires prompt and accurate diagnosis for effective management. Real-time PCR serves as the reference standard for diagnosing infection, whereas serological tests are crucial for detecting antibody responses indicative of infection or post-exposure status. Early detection during the acute phase of the disease is essential for optimal treatment and recovery.

Systematic evaluation of TaqMan real-time polymerase chain reaction assays targeting the and loci of in recombinant plasmids and naturally infected dogs.

Background And Aim: Because of the diversity of local genotypes of , genes targeted by TaqMan real-time polymerase chain reaction (RT-PCR) assays should be systematically evaluated. This study evaluated the amplification efficiency, linearity, precision, and sensitivity of two TaqMan RT-PCR assays targeting the and loci of in recombinant plasmids and naturally infected dogs.

Materials And Methods: Thirty blood samples were collected from dogs showing clinical signs of canine monocytic ehrlichiosis at the Kasetsart University Veterinary Teaching Hospital, Bangkok, Thailand.