Publications by authors named "Pedro Jose Romero"
Article Synopsis
- Standardization of protein extracts for clinical use is crucial for ensuring effective allergy testing and safety.
- The study explored a chip-based automated CE system to analyze various allergenic extracts from olive pollen used in allergy clinics.
- Results showed that this method accurately determined protein profiles, especially recognizing key allergens like Ole e 1, and proposed purified allergens as standards, though it currently does not identify specific allergens.
Background: Commercial olive pollen from uncertain cultivar origin is the common material used for clinical and biological studies. We aimed to assess the putative heterogeneity of olive cultivars with regard to the presence of the major pollen allergen Ole e 1 and to determine whether these differences have clinical relevance.
Methods: The Ole e 1 content of several cultivars was determined by immunoblotting and ultrastructural immunocytochemistry and compared to that of a commercially available olive pollen extract designed for diagnosis.