Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics.

Injectable biomaterials, such as thermosensitive chitosan (CH)-based hydrogels, present a highly translational potential in dentistry due to their minimally invasive application, adaptability to irregular defects/shapes, and ability to carry therapeutic drugs. This work explores the incorporation of azithromycin (AZI) into thermosensitive CH hydrogels for use as an intracanal medication in regenerative endodontic procedures (REPs). The morphological and chemical characteristics of the hydrogel were assessed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR).

Influence of melatonin supplementation on tissue response of endodontic sealers in Wistar rats.

Objective: This study evaluated the influence of melatonin supplementation on tissue's response of endodontic sealers in Wistar rats.

Methodology: Forty-eight rats received subcutaneous implants of four polyethylene tubes: one empty (control) and three filled with endodontic sealers (AH Plus, Endofill and Sealapex). Half of the animals were supplemented with melatonin (ME) and the remaining treated with water (WA) for 15 days before the implantation until euthanasia, forming the groups: control-WA, AH Plus-WA, Endofill-WA, Sealapex-WA, Control-ME, AH Plus-ME, Endofill-ME and Sealapex-ME.

Cytotoxicity, Biocompatibility, and Calcium Deposition Capacity of 45S5 Bioglass Experimental Paste and Bio-C Temp: In Vitro and In Vivo Study Using Wistar Rats.

The evolution of biomaterials engineering allowed for the development of products that improve outcomes in the medical-dental field. Bioglasses have demonstrated the ability to either compose or replace different materials in dentistry. This study evaluated the cytotoxicity, biocompatibility, calcium deposition, and collagen maturation of 45S5 bioglass experimental paste and Bio-C Temp, compared to calcium hydroxide (Ca(OH)) paste.

Increase Toll-like receptors 2 and 4 in apical periodontitis of rats with chronic liver disease.

The aim of this study was to evaluate the influence of liver fibrosis (LF) on the expression of Toll-like receptors (TLR) 2 and 4 in apical periodontitis (AP) in Wistar rats. Forty Wistar rats were allocated in the following groups (n = 10): C-control; AP-apical periodontitis; LF-liver fibrosis; AP + LF-rats with AP and LF. LF and AP were induced by established methodologies.

Influence of systemic antibiotic therapy on the development and progression of induced apical periodontitis in Wistar rats.

The aim of this study was to investigate the influence of systemic antibiotic therapy on the development and progression of induced apical periodontitis (AP) in Wistar rats. Fifty-six rats were submitted to pulp exposure of the lower left first molar for the induction of AP. On the same day, intraperitoneal antibiotic therapy was administered once a day, for 15 days, until euthanasia.

Influence of photoactivation on tissue response to different dyes used in photodynamic therapy and laser ablation therapy.

Laser ablation therapy (LA) uses Indocyanine Green dye (ICG) which efficiently absorbs laser energy and the increased temperature results in an instantaneous flame that chars tissue and microbes. Photodynamic therapy (PDT) uses different dyes that are activated by light to kill bacteria. This study evaluated the biocompatibility of the dye Curcumin (CUR), Methylene Blue (MB), and Indocyanine Green (ICG) before and after laser activation (ACT).

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa.

In recent years, antimicrobial peptides isolated from amphibian toxins have gained attention as new multifunctional drugs interacting with different molecular targets. We aimed to rationally design a new peptide from temporin-PTa. Hp-MAP3 (NH-LLKKVLALLKKVL-COOH), net charge (+4), hydrophobicity (0.

Evaluation of the Combination of CO Laser and Bifunctional Bonding Agents for Composite Resin Repairs After 1 Year of Aging.

to evaluate the bond strength of repairs to composite resin restorations treated with CO laser and bifunctional monomers after 1 year of aging. Adequate bond strength between a composite restoration and resin repair agent can be achieved through mechanical and/or chemical treatment. However, the longevity of such repairs is unknown.

Curcumin reduces inflammation in rat apical periodontitis.

Aim: The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP).

Methodology: Forty male Wistar rats weighing 250-280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure-induced apical periodontitis (AP) and rats with pulp exposure-induced apical periodontitis treated with curcumin (AP-CUR).

Influence of supplement administration of omega-3 on the subcutaneous tissue response of endodontic sealers in Wistar rats.

Aim: Natural substances such as omega-3 have been used in the medical field due to their numerous properties and, in particular, modulating effect on the systemic and local inflammatory processes. Thus, this study evaluated the influence of omega-3 supplementation on the subcutaneous tissue response of endodontic sealers in Wistar Rats.

Methodology: Polyethylene tubes were implanted in the subcutaneous tissue of 48 animals (one empty for control and three filled with Sealapex, AH Plus or Endofill).

Evaluation of the Effectiveness of Dental Bleaching with Carbamide Peroxide and Violet Light.

The aim of the current study was to use the CIELab system to evaluate the performance of the whitening treatment involving violet light-emitting diode (LED) combined with a home 10% and 22% carbamide peroxide dental bleaching technique on dental enamel. Fifty blocks of bovine dental enamel were divided into five groups: control group (control), receiving only LED irradiation; Whitening 10%, receiving 10% carbamide peroxide treatment; Whitening 10%+VL, receiving 10% carbamide peroxide treatment combined with LED irradiation; Whitening 22%, receiving 22% carbamide peroxide treatment; and Whitening 22%+VL, receiving 22% carbamide peroxide treatment combined with violet LED irradiation. Color tests were performed before the protocols, after 1 week and after 2 weeks of treatment by using a spectrophotometer and the CIELab parameters: L*, (a*) and (b*).

The use of omega-3 fatty acids in the treatment of oral diseases.

Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) have been reported to exert important roles in the inflammatory response. There are many inflammatory diseases in dentistry which support the administration of ω-3 PUFAs as an adjunct therapy during the treatment of these diseases. The aim of this review was to evaluate the use of ω-3 PUFAs as an adjuvant therapy during the treatment of buccal diseases.

Antimicrobial photodynamic chemotherapy mediated by PapaMBlue on chronic periodontal disease: Study protocol for a randomized, blind, controlled trial.

Background: The elimination of the pathogenic microorganisms of the periodontal pocket is one of the main points for success in periodontal treatment. The objective of this study is to investigate the clinical and antimicrobial effect of papain-mediated photodynamic therapy in the clinical treatment of periodontal disease.

Methods: Twenty patients with chronic periodontitis will be selected.

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide.

This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus.

Effect of Surface Treatment with CO Laser on Bond Strength in Composite Resin Restorations.

Evaluate the bond strength of repairs made on composite resin following the treatment of the surface of the flaw with different bonding agents and/or CO laser. The influence of CO laser and its interaction with other bonding agents on the surface of the flaw is not yet known. In this study, CO laser was chosen to treat the surface of the flaw due to its capacity to promote irregularities on the surface that enhance mechanical micro-retention.

Evaluation of Different Dentifrice Compositions for Increasing the Hardness of Demineralized Enamel: An in Vitro Study.

This study aimed to evaluate microhardness of a dentifrice containing fluoride and arginine compared to a positive control (fluoride only) and a negative control (no fluoride) on sound and demineralized bovine enamel surfaces. Specimens were randomly assigned to different treatments that included daily pH cycling and brushing three times a day with one of the following dentifrices ( = 8): Neutraçucar (arginine and fluoride), Colgate Total 12 (fluoride) and My First Colgate (no fluoride). Enamel carious lesions were artificially created one week before the beginning of these treatments (demineralized bovine enamel (DE) groups).

Microhardness of bovine enamel after different fluoride application protocols.

The aim of the present study was to evaluate microhardness, mineral recovery and the enamel surface after the application of topical fluoride to artificial dental caries. Twenty-five bovine enamel blocks were prepared for artificial caries-like lesions and randomly divided into five groups (n=5): untreated (C control), 1.23% acidulated phosphate fluoride gel (APG), 2% neutral fluoride gel (NFG), 1.

Influence of Ultrapulsed CO Laser, before Application of Different Types of Fluoride, on the Increase of Microhardness of Enamel .

Objective: To evaluate the influence of ultrapulsed CO laser in combination with commercial fluoride products in order to verify the increase of microhardness of artificial enamel caries lesions.

Materials And Methods: Bovine enamel specimens were prepared, and artificial enamel caries lesions were created. Teeth were randomly divided into 5 groups (n=10): treated with laser (L), laser + neutral fluoride gel 2% (LNF), laser + acidulated phosphate fluoride gel 1.

Bond Strength of Abraded and Non-Abraded Bleached Enamel to Resin After Er,Cr:YSGG Laser Irradiation.

Objective: The objective of the study was to evaluate the microtensile bond strength (μTBS) of a composite resin to abraded or non-abraded bleached enamel after Er,Cr:YSGG laser irradiation and to observe the fracture patterns of the tested interfaces.

Materials And Methods: Two hundred twenty-eight bovine incisors were sectioned, resulting in 228 enamel blocks (7 × 4 × 4 mm) that were divided into 12 groups (n = 19) according to the factors "adhesion" after bleaching (immediate adhesion; after 14 days; and a control group with adhesion on unbleached teeth); enamel "abrasion" (with or without abrasion simulating cavity preparation); and "laser" (with or without Er,Cr:YSGG laser irradiation). Bleached enamel groups were treated with 20% carbamide peroxide, 8 h/day for 21 days.