Publications by authors named "Pedro F Barbosa"
The secreted aspartic peptidases (Saps) of play crucial roles in various steps of fungal-host interactions. Using a flow cytometry approach, this study investigated the expression of Saps1-3 antigens after (i) incubation with soluble proteins, (ii) interaction with mammalian cells, and (iii) infection in immunosuppressed BALB/c mice. Supplementation strategies involving increasing concentrations of bovine serum albumin (BSA) added to yeast carbon base (YCB) medium as the sole nitrogenous source revealed a positive and significant correlation between BSA concentration and both the growth rate and the percentage of fluorescent cells (%FC) labeled with anti-Saps1-3 antibodies.
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- Candida species, especially Candida albicans, are the most common fungal pathogens in humans, but there’s been a rise in non-albicans Candida species (NACs) which show increased antifungal resistance.
- Recent research on a copper(II) coordination compound known as CTP has revealed its ability to effectively target and damage antifungal-resistant NACs by affecting their mitochondrial function and causing significant surface alterations.
- CTP not only inhibits biofilm formation by these invasive species but also disrupts established biofilms, making it a promising candidate for developing new antifungal treatments.
Article Synopsis
- Out of 25 tested compounds containing metal ions, 8 showed promise in inhibiting the growth of Candida albicans and Candida tropicalis, with favorable drug-like properties for topical use.
- The complex CTP demonstrated effective antifungal activity against various drug-resistant Candida species with low toxicity in animal models, suggesting its potential as a new treatment option.