Breast fat grafting and cancer: a systematic review of the science behind enhancements and concerns.

Background: Autologous fat transfer (AFT) is gaining popularity in breast surgery, offering a natural-looking and minimally invasive approach for augmentation, reconstruction, and contouring. However, concerns about its impact on breast cancer necessitate an understanding of the interplay between transplanted adipose-derived stem cells (ADSCs) and the breast tissue microenvironment. Renowned for regeneration, ADSCs raise questions about their role in cancer promotion.

Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids.

Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs.

Stability of Reference Genes during Tri-Lineage Differentiation of Human Adipose-Derived Stromal Cells.

Quantitative real-time PCR can detect variations in gene expression. The identification of the stable reference genes (RGs) is necessary to evaluate the expression of specific genes of interest under various conditions in many cell types, including human adipose-derived stromal cells (hASCs). In this study, we used the algorithms BestKeeper, NormFinder, geNorm, and RefFinder to investigate the stability of 15 potential RGs (B2M, eEF1A1, GAPDH, H2AFZ, HMBS, HPRT1, PGK1, PPIA, RPL5, SDHA, TBP, TKT, TRFC, TUBB, and UBC) in hASCs during control, adipo-, chondro-, and osteogenic differentiation for 28 days.

Tissue expander capsule for abdominal wall in autologous breast reconstruction.

Adjuvant treatment planning can change after breast cancer resection and definitive pathological examination. Radiation therapy is often chosen as a supplementary treatment. Rectus abdominis-based muscle flaps are one of the main choices when breast reconstruction plans must be changed from implant-based to autologous methods.

A gastrocnemius heterotopical transplant model with end-to-side neurorraphy.

Purpose: To present an animal model to assess the effects of end-to-side innervation in the heterotopically transplanted model with reduced chances of neural contamination.

Methods: The medial portion of the gastrocnemius muscle in wistar male rats was isolated and its pedicle dissected and performed a flap in the abdominal portion. To prevent neural contamination in the abdominal region, the muscle was wrapped with a Goretex(r) sheet.

An experimental model to retraining in microvascular suture.

Purpose: To demonstrate an experimental model of up to four hours a week of independent study that allows relearning in microvascular sutures.

Methods: Wistar rats between 200 and 500 grams surplus research experiments were used. Femoral vessels are covered on one or both sides through a groin incision obliquely along the inguinal ligament.

Rectus diastasis corrected with absorbable suture: a long-term evaluation.

Background: Correction of rectus diastasis (RD) is performed during most abdominoplasties. This study aimed to evaluate the long-term result of RD correction when the plication of the anterior rectus sheath is performed with an absorbable suture.

Methods: Abdominoplasty was performed for 12 women who presented with Nahas' type A musculoaponeurotic deformity.

Nicotine on rat TRAM flap.

Purpose: The transverse rectus abdominis myocutaneous (TRAM) flap is one of the preferential techniques used in breast reconstruction following mastectomy. Nicotine has a detrimental effect on cutaneous flap survival; although there are no experimental studies proving this effect on musculocutaneous flaps. The aim of this study is to verify the effect of nicotine on the rat TRAM flap.

Tensile strength of the posterior and anterior layer of the rectus abdominis muscle sheath in cadavers.

Purpose: The aim of the present study was to assess, in cadavers, the resistance to medial traction of the posterior and anterior layer of the rectus abdominis muscle sheath.

Methods: An anatomical, prospective, self-controlled study was carried out in 20 male cadavers. An evaluation of the tension of the posterior and anterior layers of the rectus abdominis muscle sheath was performed following anatomical dissection of these structures.

Ultrasonography for measuring rectus abdominis muscles diastasis.

Purpose: Preoperative imaging evaluation may be useful for determining the position of recti abdominis muscles before their correction. The purpose of this study is to evaluate the accuracy of ultrasonography to measure the width of rectus abdominis muscle diastasis.

Methods: Rectus diastasis was measured by ultrasonography preoperatively in 20 females.