Cytotoxicity of silver and copper nanoparticles on rainbow trout (Oncorhynchus mykiss) hepatocytes.

Nanoparticles are commonly used in the industry and are present in consumer goods; therefore, evaluation of their potential toxicity is necessary. The aim of the present study was to assess the cytotoxic effects of the nanoparticles of silver (AgNPs) at the concentration of 1.5 mg L and copper (CuNPs) at 0.

The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells.

Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane.

Glioblastoma multiforme - an overview.

Glioblastoma multiforme is a central nervous system tumor of grade IV histological malignancy according to the WHO classification. Over 90% of diagnosed glioblastomas multiforme cases are primary gliomas, arising from normal glial cells through multistep oncogenesis. The remaining 10% are secondary gliomas originating from tumors of lower grade.

Proliferative and apoptotic activity of glioblastoma multiforme cells cultured on in ovo model.

Aim: The influence of antitumor compounds on glioblastoma cell activity can be successfully investigated on an in ovo model. To consider an in ovo model as a reliable tool for estimation of anticancer drug efficacy, the biological activity of tumors growing in such conditions should be comparable to spontaneous cases. The aim of the present study was to evaluate the biological characteristics of glioblastoma multiforme (GBM) tumors - defined as their proliferative and apoptotic activities - growing on an in ovo model.

[Regulation of apoptosis in endometrium preparation for menstruation or embryo implantation].

Recent studies have presented apoptosis as the key mechanism of endometrial tissue reconstruction. Regulation of apoptosis leads to the menstruation or prepare the mucose layer for the implantation of the embryo. Thus, the factors controlling apoptosis determine proper endometrial preparation for blastocyst implantation.

[Chorionic gonadotropin as the key factor for embryo implantation].

Chorionic gonadotropin (CG), as a embryonic factor, controls the implantation process and pregnancy formation. The hormone regulates endometrial functions by its influence on progesterone production and direct modulation of endometrial cell functions. Recent studies suggest the key role of CG in controlling the main implantation processes like apoptosis, angiogenesis, maternal immunological response and trophoblast migration.

Influence of hydrocolloidal silver nanoparticles on gastrointestinal microflora and morphology of enterocytes of quails.

The objective of the present study was to examine the effects of hydrocolloidal silver nanoparticles (Ag-nano) on microbial profile of caecum and morphology of enterocytes in duodenum of Japanese quail, as a model animal for poultry. Quails (Coturnix coturnix japonica) (10 d old) were randomly divided into four groups (15 quails each) and located into four cages for 12 days. Quails were fed with granulated diets given ad libitum and had free access to drinking water.

Epididymal cysts in European bison.

We describe gross and histopathology of cysts found in the epididymis of the European bison (Bison bonasus). The material was collected from 107, 3 mo to 20 yr old, free-ranging, culled males from the Białowieza Primeval Forest (Poland). Epididymal cysts were observed in 65 (61%) of bison of varying ages.

Reproductive performance of a transgenic boar carrying the bovine growth hormone gene (bGH).

The aim of this study was to obtain information on the possible influence of the bovine growth hormone gene (bGH) on gametogenesis and reproductive parameters of a 2-year-old Polish Landrace transgenic boar. The bGH gene construct had been introduced into the zygote of the boar with the use of the microinjection technique. On the basis of the available documentation we established that the fertility of the investigated transgenic (bGH) boar was low in comparison with other animals of the Polish Landrace breed, with a poor libido, ineffective matings and, on average, 3 live piglets less per litter.