Application of micro-TLC to the total antioxidant potential (TAP) measurement.

DPPH is commonly applied for estimation of antioxidant capacity of single and complex biological samples, and changes colour from purple to yellow during reduction to DPPH-H. Importantly, for some samples, for example coloured foods, such reaction cannot be used because of interference from pigments. Therefore, the number of reported quantitative protocols involving off- or on-line sample reaction with DPPH are based on chromatographic separation of target components.

A fast and simple method for the measurement of total antioxidant potential and a fingerprint of antioxidants.

The goal of this paper is to demonstrate a new approach to the chromatographic total antioxidant potential (TAP) assay and chromatographic fingerprints of honeys. The analyte is analyzed using reversed-phase high-performance liquid chromatography with electrochemical (amperometric) detection. The TAP measure was the total surface area of all recorded chromatographic peaks (on the amperometric detector in the oxidation potential range) on the chromatogram.