Publications by authors named "Pawel Gluza"
Yeast-two-hybrid (Y2H) is widely used as a strategy to detect protein-protein interactions (PPIs). Recent advancements have made it possible to generate and analyse genome-wide PPI networks en masse by coupling Y2H with next-generation sequencing technology. However, one of the major challenges of yeast two-hybrid assay is the large amount of false-positive hits caused by auto-activators (AAs), which are proteins that activate the reporter genes without the presence of an interacting protein partner.
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- Glycosyltransferases (GTs) are crucial enzymes for forming glycosidic linkages, involved in producing various important biological products like glycans and glycoproteins, and plants have a significantly larger variety of these enzymes compared to animals.
- This study enhances the existing GT clone collection by adding 105 new GT cDNAs, resulting in a total of 508 clones, and establishes a comprehensive pipeline for analyzing these enzymes' functions using techniques like mass spectrometry and enzymatic assays.
- Focusing on the GT14 family, the research successfully demonstrated the activity of specific GT enzymes in synthesizing β-glucuronosyltransferase and identified a new member with greater activity, suggesting
Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and plant-defense responses. Despite the importance of pectic polysaccharides their biosynthesis is poorly understood. Several genes have been implicated in pectin biosynthesis by mutant analysis, but biochemical activity has been shown for very few.
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