Publications by authors named "Pavlovich E"

Aim: To study effects of intravenous infusion of a cardioprotective drug metilin, developed at the "National Medical Research Center of Cardiology" on indices of cardiac function in rabbits in vivo after prolonged administration of doxorubicin.

Materials And Methods: Animals of the experimental group were intravenously injected with doxorubicin (2 mg / kg once a week) for 8 weeks, animals of the control group received the same volume of saline. Myocardial damage was characterized by an increase in concentration of malondialdehyde (MDA), troponin (TnI) and MB-fraction of creatine kinase (CK-MB) in venous blood and by disturbances in the left ventricle (LV) structure at morphological examination.

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Cell culture models are excellent tools for potential toxicity of nanoparticles and fundamental investigations in cancer research. Thus, information about AuNP potential toxicity and effects on human health is necessary for the use of nanomaterials in clinical settings. The aim of our research is to examine the effects of AuNPs on the epithelial origin cell lines: continuous and oncogenic.

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Exposure of human cryopreserved spermatozoa to millimeter-wave electromagnetic radiation of 0.03 mW/cm2 density for 5 min in normozoospermia and for 15 min in asthenozoospermia lead to increase of the fraction of mobile spermatozoa without impairing the membrane integrity and nuclear chromatin status and without apoptosis generation.

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The effects of a single intravenous injection of human umbilical blood were studied on the model of severe spinal cord contusion injury in rats. Rats receiving no umbilical blood (spontaneous recovery) served as the control. All rats exhibited pronounced hind limb paraplegia and autonomic dysfunction of pelvic organs after the injury.

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Ultrastructure of working myocardium was studied in rabbits subjected to 70-day immobilization in narrow cages excluding active movements. The structure of working cardiomyocytes, nervous apparatus, and microvascular bed in auricles were studied. Particular attention was focused on intercalated disks in muscle fibers, where separation of fasciae adherents was observed, while desmosomes in the lateral area of these contacts were preserved.

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The EM study of capillaries in the sinus node (SN) and adjacent working myocardium has been performed in 12 patients who ceased a sudden coronal cardiac death at the age 34-69 years and in 8 patients with alcoholic cardiomyopathy who suddenly died at the age 32-60 years. Quantitative evaluation of the density of the capillaries and their diameters in the myocardium of this region was made. Acute and chronic changes were observed in the capillaries of SN and working myocardium.

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Ultrastructural investigation of cellular contacts of sinus node (SN), conducting myocytes of types I and II and working myocytes of right atrium (RA) perinodular myocardium was performed in rat heart. Lateral contacts between myocytes and intercalated discs are the basic in this area. Desmosomes and nexuses were found in lateral contacts apart from regions of simple abutment of plasmalemma.

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A qualitative ultrastructural analysis of specialized conducting and working myocytes in the sinoatrial region of the human heart was made using autopsied and biopsied material from 45 men and women. Autopsied material was prepared not later as than 3 hours after the death. Biopsied material was taken during the operation for surgical correction of disturbances of cardiac rhythm and conduction.

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The EM study of all tissues and myocyte components in the sinus node (SN) and adjacent working myocardium of the right atrium was performed in normal dogs and in dogs 1, 2, and 3 hours after death. A quantitative evaluation of the myocyte density, connective tissue components, vessels and nerve fibers in the SN and working myocardium of this region was performed. Destructive changes having mosaic appearance were observed in the conducting and working myocytes, myelinated and non-myelinated, nerve fibers, in vessels and partly in components of connective tissue of both SN and working myocardium.

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Ultrastructural analysis of cells, collagen and elastic fibres and connective tissue matrix was performed separately in sinoatrial node (SN) and the right atrium (RA) perinodal working myocardium in 12 patients of different age with idiopathic long Q-T syndrome. Despite the variability in SN and RA connective tissue component content in different patients these parameters were often significantly different for working and conducting myocardium. In senior group SN and RA elastic fibres content showed 1.

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All nerve components of the sinus node (SN), adjacent working myocardium and nervous ganglions of 12 patients, from 9 to 50 years old, with an idiopathic syndrome of a long Q-T interval were studied electron-microscopically. Quantitative evaluation of the myocardium innervation density in this heart region was performed. Destructive changes of the myelinated and non-myelinated nervous fibers and gliocytes in the conductive myocardium of SN and working myocardium as well as in the neurocytes of the adjacent nervous ganglions were observed.

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The quantitative and qualitative ultrastructural analysis of specialized conducting and working myocytes in papillary muscles of the rabbit heart was made. The volume fractions of myofibers, mitochondria, nuclei, lipids, vacuoles, glycogen, sarcoplasmatic reticulum, T-tubules, lysosomes, and "clear" cytoplasm of conducting and working myocytes were estimated in addition to cell diameters. The results were compared with literature data for the Purkinje cells of different mammals.

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The EM study of the nerve components of the sinus node, adjacent contractile myocardium and nerve ganglions has been performed in 9 suddenly deceased males with alcoholic cardiomyopathy. A quantitative evaluation of the innervation density in the myocardium is given. Destructive changes were observed in the myelinized the non-myelinized nerve fibers of both conductive and contractile myocardium as well as in adjacent to the node nervous ganglions and their neurocytes and glia.

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The paper deals with the idiopathic prolonged QT interval syndrome, a rare and highly malignant cardiac disease, which is characterized by ECG changes, episodes of polymorphic torsades de pointes ventricular tachycardia, and high family history mortality due to sudden cardiac arrest. The causes and optimum treatments of the disease remain less studied. The authors propose noninvasive techniques for investigating the functional characteristics of the cardiac conduction system, whose use is highly important both for the understanding of the nature of the available changes and the diagnosis of asymptomatic types of the disease course.

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Quantitative electron microscopic study of the innervation of different types of specialized sinus node conducting myocytes and of working myocytes in the perinodal myocardium of the right atrium was carried out in intact dogs. Differences were found in diameters of unmyelinated nervous fibers and their efferent terminals and distances between them and sarcolemmas of myocytes of the types studied. It was demonstrated that qualitatively different synaptic vesicles in the efferent terminals near the nodal and working myocytes are encountered with different frequency.

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Sinus nodes of five symptomatic patients with the long QT syndrome were surgically excised and followed by permanent electronic pacing as part of a new surgical treatment. We examined those sinus nodes by light and electron microscopy with tissue that was promptly fixed at the time of surgery. All five sinus nodes were similarly abnormal.

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The tissue components of the conducting myocardium of the sinus node (SN) and the working prenodal myocardium of the right atrium (RA) in the sinoatrial area of the heart were quantitatively ultrastructurally analyzed in 8 males who had suddenly died from alcohol cardiomyopathy with (n = 3) or without acute alcohol intoxication at the age of 32 to 56 years. The volume densities of myocytes, connective tissue, capillaries and nerves, as well as the diameters of conducting and working myocytes were measured individually for SN and RA. The findings were compared with those in sudden extracardiac death and sudden coronary death.

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Light and electron microscopic studies of nervous ganglia of the sinoauricular area of the heart were performed in 7 people dead at the age from 23 to 58 years (44 +/- 6) from not cardiac causes. The ganglia were disposed under the epicardium and had from 1 to 17 neurons as well as bundles of myelinated (MNF) and nonmyelinated nerve fibers (NNF). The ganglia most often had light neurons with a rounded nucleus and a compact nucleolus, with invariable cisterns of the granular endoplasmic reticulum microfilaments, electron-dense bodies, lipofuscin granules and edematous mitochondria.

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Neurohistochemical and electron microscopic investigations of the autonomic nervous system (ANS) of man and animals suggest that its ontogenesis can be divided into the premediatory, mediatory and postmediatory periods of development. The postmediatory period begins heterochronically in various ganglia of the ANS. A normal process of early cardiac desympathization usually occurs at the age of 35 to 60 years.

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A quantitative ultrastructural analysis was made of tissue components in the conducting myocardium of the sinus node and in the working perinodal myocardium of the right atrium from the sinoauricular region of the heart in 10 subjects who had suddenly died of coronary heart disease or cerebral hemorphage in the presence of hypertensive disease. Volumetric densities were estimated in myocytes, connective tissue, capillaries, and nerves; diameters of conducting and working myocytes were also measured in the sinoauricular region of the heart. The data of the analysis were compared with those of estimation of tissue components in the sinoauricular region in sudden extracardiac death.

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Qualitative and quantitative electron microscopic analysis of collagenous fibers has been performed in the sinusal node (SN) and perinodal working myocardium of the right atrium (RA) in the hearts of 22 men, died at the age of 23-63 years. Five of them died from alcoholic cardiomyopathy, 8--from coronary cardiac disease and 9--from noncardiac causes and craniocerebral trauma (group of comparison). In 21 cases seven types of ultrastructural changes of the collagenous fibers have been revealed in the SN (changes in the form, size and degree of their osmiophilia).

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A quantitative electron microscopical examination of specialized conducting myocytes of types II and III in the atrioventricular node and working myocytes in perinodal region was performed. The volume fractions of myofibrils, mitochondria, nuclei, sarcoplasmic reticulum, glycogen, atrial granules and "clear" cytoplasm were measured, in addition to the diameters of conducting and working myocytes. These data were compared with those for conducting myocytes of types I, II, and III from sinoatrial region and internodal tracts of the rat heart, and with evidence for atrioventricular node of other animals.

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The conducting myocardia of the sinus node and prenodal working ones of the right atrium, which had been dissected from 6 patients aged 10-40 years with the prolonged Q-T interval syndrome during an operation on implantation of an artificial pacemaker were examined. Acute and chronic destructive changes were found in the conducting and working myocytes of the sinoauricular region in the heart. Some cases displayed abnormal contact interrelationships of myocytes.

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An electron-microscopic study of the sinus nodes (SN) was conducted in the hearts of 8 males aged 32-60 who had suddenly died of alcoholic cardiomyopathy in the background of acute alcohol intoxication (4 cases) or without it (4 cases). The hearts of matched-for-age 7 males and 1 female whose cause of sudden death was other than cardiac served as control. It appeared that all the components of the sinus nodes from hearts of the study group underwent chronic or acute destructive changes.

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Light- and electron-microscopic studies were performed on hearts from 23 males (aged 34 to 69 years) who had died suddenly with coronary heart disease. The hearts from 6 males of the same age group who had succumbed to extracardiac causes (including injuries) served as controls. Chronic abnormalities and acute destructive changes in the sinus node were observed in 82.

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