Simplified Method for -Mediated Genetic Transformation of x K. Koch.

The rapid advancement of genetic technologies has made it possible to modify various plants through both genetic transformation and gene editing techniques. Poplar, with its rapid growth and regeneration enabling high rates of micropropagation, has emerged as a model system for the genetic transformation of woody plants. In this study, K.

Changes of cellular stress response related and transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range.

The induction of cellular stress response systems, heat shock protein /Hsp70 and multixenobiotic transporter , by cadmium chloride (CdCl) was explored in amphipod species with different stress adaptation strategies from the Lake Baikal area. Based on the lethal concentrations (LC) of CdCl, the sensitivities of the different species to CdCl were ranked (24 hr LC50 in mg/L CdCl (mean/95% confidence interval)): (1.7/1.

Use of a combined effect model approach for discriminating between ABCB1- and ABCC1-type efflux activities in native bivalve gill tissue.

Aquatic organisms, such as bivalves, employ ATP binding cassette (ABC) transporters for efflux of potentially toxic chemicals. Anthropogenic water contaminants can, as chemosensitizers, disrupt efflux transporter function enabling other, putatively toxic compounds to enter the organism. Applying rapid amplification of cDNA ends (RACE) PCR we identified complete cDNAs encoding ABCB1- and ABCC1-type transporter homologs from zebra mussel providing the molecular basis for expression of both transporter types in zebra mussel gills.

Identification of a putatively multixenobiotic resistance related Abcb1 transporter in amphipod species endemic to the highly pristine Lake Baikal.

The fauna of Lake Baikal in Eastern Siberia, the largest freshwater body on Earth, is characterized by high degrees of biodiversity and endemism. Amphipods, a prominent taxon within the indigenous fauna, occur in an exceptionally high number of endemic species. Considering the specific water chemistry of Lake Baikal with extremely low levels of potentially toxic natural organic compounds, it seems conceivable that certain adaptions to adverse environmental factors are missing in endemic species, such as cellular defense mechanisms mitigating toxic effects of chemicals.

Contrasting cellular stress responses of Baikalian and Palearctic amphipods upon exposure to humic substances: environmental implications.

The species-rich, endemic amphipod fauna of Lake Baikal does not overlap with the common Palearctic fauna; however, the underlying mechanisms for this are poorly understood. Considering that Palearctic lakes have a higher relative input of natural organic compounds with a dominance of humic substances (HSs) than Lake Baikal, we addressed the question whether HSs are candidate factors that affect the different species compositions in these water bodies. We hypothesized that interspecies differences in stress defense might reveal that Baikalian amphipods are inferior to Palearctic amphipods in dealing with HS-mediated stress.

Expression patterns and organization of the hsp70 genes correlate with thermotolerance in two congener endemic amphipod species (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal.

We studied various aspects of heat-shock response with special emphasis on the expression of heat-shock protein 70 (hsp70) genes at various levels in two congener species of littoral endemic amphipods (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal which show striking differences in their vertical distribution and thermal tolerance. Although both the species studied demonstrate high constitutive levels of Hsp70, the thermotolerant E.

Evaluation of biochemical responses in Palearctic and Lake Baikal endemic amphipod species exposed to CdCl2.

This study evaluated small heat shock proteins (sHSP) (related to alpha-crystallin) and antioxidant enzymes (POD, peroxidase and CAT, catalase) as possible biomarkers for use in toxicological studies. Biochemical responses to cadmium chloride in two Lake Baikal endemic amphipods (Eulimnogammarus verrucosus, Eulimnogammarus cyaneus) and Palearctic species (Gammarus lacustris) were compared. Our findings showed that cadmium chloride toxicity directly influenced POD activity and sHSP synthesis in all amphipod species.

[New subspecies of black flies Chelocnetha angustitarsis zaporojae ssp. n. (Simuliidae) from the steppe zone of the Ukraine].

A new subspecies of simuliids, Chelocnetha angustitarsis zaporojae Pavlichenko ssp. n., from the steppe zone of the Ukraine is described.

[Variability of postvaccinal antibody activity in swine with different blood groups].

In pigs with A--/--, Kace/--, Kace/b; Lbdfi/bdfi-allels in the genotype the titre of postvaccinal antibodies was 4-15 times as high as in pigs with allels Acp; Lbcgi/adhi, Kb/-or K--/--. Frequency of animals with allels, connected with a low titre of antibodies in certain populations ranged from 0 to 11.6%.