Fluorescence attenuated by a thick scattering medium: Theory, simulations and experiments.

Fluorescence-based imaging has an enormous impact on our understanding of biological systems. However, in vivo fluorescence imaging is greatly influenced by tissue scattering. A better understanding of this dependence can improve the potential of noninvasive in vivo fluorescence imaging.

Diffused reflectance measurements to detect tattoo ink location in skin using the crossover point.

Tattoos are highly trendy in western culture, but many people regret their tattoos for many reasons. It is essential to be aware of the ink location in advance to reduce the long and short-term side effects. In this study, diffuse reflectance (DR) experiments were conducted on two-layer (2L) tissue-mimicking phantoms, where ink was sandwiched between the layers.

Bottom layer absorption coefficients extraction from two-layer phantoms based on crossover point in diffuse reflectance.

Significance: Numerous optical imaging and spectroscopy techniques are used to study the tissue-optical properties; the majority of them are limited in information regarding the penetration depth. A simple, safe, easily applicable diagnostic technique is required to get deeper tissue information in a multilayer structure.

Aim: A fiber-based diffuse reflectance (DR) technique is used to extract and quantify the bottom layer absorption coefficients in two-layer (2L) tissue-mimicking solid phantoms.