Effects of immobilizations stress with or without water immersion on the expression of atrial natriuretic peptide in the hearts of two rat strains.

Atrial natriuretic peptide (ANP) is produced and released by mammalian cardiomyocytes and induces natriuresis, diuresis, and lowering of blood pressure. The present study examined localization of ANP and a possible role of the hypothalamic-pituitary-adrenal axis (HPA) activity on the expression of proANP gene in the heart. The Sprague Dawley (SD) and Lewis (LE) rat strains were used.

Effects of acute stressors on the expression of oxytocin receptor mRNA in hearts of rats with different activity of HPA axis.

Objectives: Cardiovascular system is regulated by a diverse array of hormones, neurotransmitters and neuropeptides. Oxytocin and its receptors (OTR) were also shown to regulate cardiovascular functions and this hormone was even called cardiovascular hormone. In recent publication, we demonstrated the expression of mRNA of OTR by real-time quantitative PCR (RT qPCR) in all rat heart compartments.

Expression of heart oxytocin receptor and its mRNA in two rat strains with different activity of HPA axis.

Objectives: Oxytocin (OT) is a neuropeptide acting both as a peripheral hormone and in the brain as neurotransmitter and neuromodulator. In addition to its well-known effects on milk-ejection and uterine contraction, OT was shown to exert neuroendocrine regulation of heart functions. The aim of this study was to investigate the expression of mRNA of OT receptors (OTR) in rat hearts by real-time quantitative PCR (qPCR).

Galanin modulating effect on restraint stress-induced short- and long-term behavioral changes in Wistar rats.

The neuropeptide galanin has been recognized as a possible neurotransmitter/neuromodulator, and in addition has been implicated in anxiety- and depression-related behaviors. The present study demonstrates increased locomotion and rearing after galanin (0.3mg/kg) that was given intraperitoneally (i.

Oxytocin and carbetocin ameliorating effects on restraint stress-induced short- and long-term behavioral changes in rats.

Objectives: Carbetocin (CBT), an oxytocin (OXY) analog, was designed to exert prolonged peripheral actions. It has also been proposed as potential therapeutic mean in certain psychiatric disorders where OXY role has been implicated. This study examined the effects of both peptides on behavior of naive and restraint stress exposed rats in the open field (OF) and elevated plus maze (EPM) tests.

Oxytocin and carbetocin effects on spontaneous behavior of male rats: modulation by oxytocin receptor antagonists.

Objectives: Oxytocin (OXY) in addition to peripheral actions has many central regulatory functions which can be studied on animal models. In the present study we examined in rats, which behavioral actions of OXY and long-acting carba-analog of OXY carbetocin (CBT) in the open-field can be inhibited by OXY-receptor antagonists. Our interest focused on the behavioral patterns considered indicative of anxiety-related behavior.

Effects of melanotan II, a melanocortin agonist, on grooming and exploration in rats after repeated restraint/immobilization.

2mg/kg melanotan II (MTII, administered i.p.), a cyclic peptide analog of alpha-melanocyte stimulating hormone, at a single dose increased grooming in naive rats placed in an unfamiliar open-field device without changing locomotion or rearing.

Timing of stress and testing influence the long-lasting behavioral performance in rats.

Three exposures (Days 1, 2 and 3) of rats to immobilization or immobilization combined with cold induced an alteration of exploratory behavior in an open space arena. When tested 1h after both stressors exposure, rats displayed a decrease in locomotor and rearing score. The deficit disappeared when rats were tested five days later and the performance remained unchanged in trials performed on days 9, 10, 15, 22 and 29 of the study.

Differences in the brain expression of c-fos mRNA after restraint stress in Lewis compared to Sprague-Dawley rats.

In order to study the contribution of genetic factors to the pattern of stress-induced brain activation, we studied the expression of c-fos mRNA, a marker of neuronal activity, in male Sprague-Dawley and Lewis strains, the latter being known to have a deficient responsiveness of the hypothalamic-pituitary-adrenal (HPA) axis. Immobilization (IMO) alone or combined with the immersion into water at 21 degrees C was applied for 15 or 60 min. The expression of c-fos mRNA was quantified by in situ hybridization in those brain areas that represent important parts of neuronal circuits activated by stress: medial prefrontal cortex, medial amygdala, lateral septum ventral part, paraventricular nucleus of the hypothalamus and locus coeruleus.

Effects of two types of restraint stress on the spontaneous behaviour in rats.

Our previous findings suggested the existence of stressor-specific behavioural and cognitive responses in rats. In the present study, restraint stressor (immobilization, IMO) and restraint stressor combined with partial immersion of rats into water (IMO+C) were applied for 1 hour to Wistar male rats and their spontaneous behaviour was examined in the open field test. The classic behavioural parameters were recorded: crossing, rearing, and resting.

Effects of two types of restraint stress on the learned behaviour in rats.

To study the effects of stress on cognitive functions, Wistar and Lewis rats were exposed to restraint (immobilization stressor) (IMO) or restraint combined with partial immersion into water (IMO+C). Learned discriminatory avoidance response in Y-maze, with foot-shock as an unconditioned stimulus, was used as a memory test. The latency to enter the correct arm and number of wrong entries were daily recorded during the training period (20 days) until the criterion was reached, which was set at 90% avoidances (choosing the correct arm).

Combined restraint and cold stress in rats: effects on memory processing in passive avoidance task and on plasma levels of ACTH and corticosterone.

The effect of restraint stress combined with water immersion (IMO+C), applied at various intervals before and after the acquisition of a passive avoidance task, was studied in rats. The procedure started with two pre-training trials. On the single training trial the rats received a footshock (0.

Impaired passive avoidance acquisition in Sprague-Dawley and Lewis rats after restraint and cold stress.

The study examined the effects of restraint combined with cold water stress (IMO+C) on learning and memory of Sprague-Dawley (S-D) and Lewis (LE) rats in the passive avoidance task. The procedure started with 6 days of adaptation to the apparatus during which the recorded latencies to enter the dark compartment were used to assess the process of habituation. On the training day rats were exposed to IMO+C for 60 min and the stressor exposure terminated 1 h before the acquisition trial.