Experimental and Natural Infections of Tick-Borne Encephalitis Virus in Dogs.

Dogs are frequently infected with the tick-borne encephalitis virus (TBEV). However, to date, only a few clinically manifest cases of tick-borne encephalitis (TBE) have been reported in dogs. In this study, three-month-old beagle dogs were infected with TBEV through a subcutaneous injection.

Seroprevalence of Antibodies against s. l. and s. l. in Cats in district of Brno and its environs, the Czech Republic.

Objectives: The aim of this study is to evaluate the seroprevalence of antibodies of sensu lato (sl) and sensu lato (sl) and their possible concurrence in domestic cats living in variable conditions in South Moravia in the district of Brno and its environs. Additional objectives were to discover possible differences in seroprevalence between groups of cats living in different living conditions, and to determine the spectrum of Leptospira serogroups in cats in the same places.

Material And Methods: A total of 360 blood sera from domestic cats of 3 different sets were collected during the period 2013-2015.

Tick-borne encephalitis in dogs: application of "nested real-time RT-PCR" for intravital virus detection.

Tick-borne encephalitis (TBE) virus is a tick-transmitted virus causing disorders of the nervous system in humans, monkeys, dogs and horses (rarely). At present the detection of TBE infection in dogs is performed by confirmation of seroconversion in paired samples of serum in clinical practice. The intention of the study was the assessment of the possible application of nested real-time RT-PCR for detection of TBE virus in canine blood.

Whole genome sequencing and phylogenetic analysis of feline anelloviruses.

Torque teno felis virus (FcTTV) was detected in the cat population in the Czech Republic. A total of 110 serum samples were tested by a nested PCR technique using specific primers, situated in the highly conserved untranslated region of the virus genome. The frequency of feline TT virus in the Czech Republic was found to be 33.

Detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in dogs in the Czech Republic.

The aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with Anaplasma phagocytophilum or Borrelia burgdorferi sensu lato (s. l.) in the Czech Republic.