75-Year dynamics of the Black Sea phytoplankton in association with eutrophication and climate change.

Based on a database containing species records obtained from 1948 to 2022 and a hydrochemical database, long-term changes in the biomass and taxonomic structure of phytoplankton in the deep-sea basin of the Black Sea were analysed in the stratified period from April to October. Over 75 years, a significant increase in concentration of nitrate, a weak increase in phosphate and a strong decrease in dissolved silicate were observed in the nutricline. The biomass of diatoms and total phytoplankton increased several times during the peak of eutrophication in 1991-1993, then decreased by the 2000s and has again shown an increasing trend in the last 15 years.

An impact of non-native species invasions on the Caspian Sea biota.

The Caspian Sea is a large inland brackish basin, vulnerable to invaders due to its long isolation and considerable endemism among its native biota. A brief description of Caspian biota evolution until its modern state is given. The pathways and vectors of invasion and the ways of establishment of non-native species since the early 20th century are summarized.

Dataset of the abundance and phytoplankton composition in the Barents Sea in summer 2014-2018.

This data article contains data on the abundance, phytoplankton composition, in the Barents Sea in summer 2014-2018, and physical and hydrochemical parameters in summer 2017. The data are based on the samples collected on stations, where blooms were recorded. The physical data included the water temperature, salinity, oxygen concentrations at the surface, and various depths.

Interannual variability of Emiliania huxleyi blooms in the Barents Sea: In situ data 2014-2018.

Using in situ data of 2014-2018, the summers Emiliania huxleyi blooms in the Barents Sea were studied. The blooms were recorded in the upper mixed layer in July and August every year, during which they spread to cover large areas and were associated with Atlantic waters. The E.

Drivers of phytoplankton blooms in the northeastern Black Sea.

In order to understand of the processes controlling phytoplankton successions in the NE Black Sea, long-term data series are needed. We compiled 15 years (2002-2017) of measurements from which the existence emerges of a tight link between phytoplankton species dominance and nutrients concentrations. The latter is strongly influenced by wind direction.

[A study of dissolved oxygen flux across the water-bottom interface in the northeastern Black Sea (in the region of Dzhubga village)].

Oxygen exchange at the water-bottom interface in the northeastern Black Sea was studied using bottom tanks (fluxes and oxygen consumption for organic matter mineralization and for respiration of soil and water organisms). The relationship of biogenic fluxes and patterns of biochemical (enzyme) destruction of organic matter by the components of the bacterial and microproducer community was established. The prevalence of microbial oxidation of organic matter correlated with high proteolytic activity in near-bottom water.

[Study of the oligonucleotide binding sites on an immunoglobulin molecule].

Interaction of IgG molecules with oligonucleotides using reactive derivatives of p(T)16 bearing a 4-[(N-2-chloroethyl-N-methyl)amino]benzylamine residue at the 5'-terminal phosphate was investigated. The modified immunoglobulins were degraded with pepsin into Fab and Fc fragments and by incomplete CNBr hydrolysis into smaller peptides. It became obvious from the analysis of the peptides obtained that the oligonucleotides contacted with immunoglobulins at the antigen-binding Fab fragment of the molecule.

Serum immunoglobulins interact with oligonucleotides.

The interaction of reactive derivatives of oligonucleotides bearing a 4-[(N-2-chloroethyl-N-methyl)amino]benzylamin residue at the 5'-terminal phosphate with serum blood proteins has been investigated. It was found that the compounds react with serum albumin and immunoglobulins M and G, the reactivity increasing in the order: albumin < IgG < IgM. The reactions with immunoglobulins were inhibited in the presence of different oligonucleotides, DNA and heparin, suggesting the oligonucleotide binding to some cationic region of the proteins.

[Interaction of oligonucleotides with blood serum proteins].

The interaction of alkylating derivatives of deoxyribonucleotides whose 5'-terminal phosphate group contains a 4-[(N-2-chloroethyl-N-methyl)amino]benzylamine residue with serum proteins has been studied. Incubation of whole human sera with various concentrations of the alkylating derivative, p(T)16, resulted in affinity modification of several proteins, among which albumin as well as IgM and IgG were the most readily detectable ones. The type of dependence of the degree of modification of these proteins on oligonucleotide concentration suggests that the oligonucleotides display a higher affinity for IgM than for IgG and albumin.