Cold-Active Lipase from the Ice Cave SC65A.3 Strain, a Promising Biocatalyst for Silybin Acylation.

Cold-active lipase from the psychrophilic bacterial strain SC65A.3 isolated from Scarisoara Ice Cave (Romania) was cloned and characterized as an extremophilic biocatalyst for silybin acylation. Structural analyses highlighted conserved motifs confirming a functional lipase and the presence of primary structure elements for catalysis at low temperatures.

Acoustic Fractional Propagation in Terms of Porous Xerogel and Fractal Parameters.

This article portrays solid xerogel-type materials, based on chitosan, TEGylated phenothiazine, and TEG (tri-ethylene glycol), dotted with a large number of pores, that are effectively represented in their constitutive structure. They were assumed to be fractal geometrical entities and adjudged as such. The acoustic fractional propagation equation in a fractal porous media was successfully applied and solved with the help of Bessel functions.

AI Evaluation of Imaging Factors in the Evolution of Stage-Treated Metastases Using Gamma Knife.

Background: The study investigated whether three deep-learning models, namely, the CNN_model (trained from scratch), the TL_model (transfer learning), and the FT_model (fine-tuning), could predict the early response of brain metastases (BM) to radiosurgery using a minimal pre-processing of the MRI images. The dataset consisted of 19 BM patients who underwent stereotactic-radiosurgery (SRS) within 3 months. The images used included axial fluid-attenuated inversion recovery (FLAIR) sequences and high-resolution contrast-enhanced T1-weighted (CE T1w) sequences from the tumor center.

Mercury Bonding to Xerogel: The Interface Fractal Dynamics of the Interaction between Two Complex Systems.

This study describes novel solid substances founded on chitosan and TEGylated phenothiazine that have a high ability for hydrargyrum recovery from watery liquid solutions. These compounds were taken into account, consisting of two distinct entity interactions inside of the classic fractal dynamics conjecture of an "interface". They were assimilated through fractal-type mathematical objects and judged as such.

Fractal Analysis of Four Xerogels Based on TEGylated Phenothiazine and Chitosan.

The present article describes novel massive materials (in the solid phase) based on TEGylated phenothiazine and chitosan that possess great capability to recover mercury ions from constituent aqueous solutions. These were produced by chitosan hydrogelation accompanied by formyl subsidiary item of TEGylated phenothiazine, attended by lyophilization. The delineation and structure description of the obtained material or supramolecular assembly were realized by FTIR (Fourier transform infrared) spectroscopy, X-ray diffraction, and POM (Polarized Light Optical Microscopy).

Gels: Synthesis, Characterization and Applications in High Performance Chemistry.

Organogels, hydrogels, and ionic gels are investigated both theoretically and experimentally [...

Xerogels Morphology Details by Multifractal Analysis and Scanning Electron Microscopy Images Evaluations of 5-Fluorouracil Release from Chitosan-Based Matrix.

Four medicament delivery formulations based on 5-fluorouracil in a chitosan substantial matrix were realized in situ via 3,7-dimethyl-2,6-octadienal element hydrogelation. Representative samples of the final realized compounds were investigated from an analytic, constitutional, and morphological viewpoint via Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The SEM images of the formulations were investigated in concordance with fractal analysis, and the fractal dimensions and lacunarity were computed.

A Multifractal Vision of 5-Fluorouracil Release from Chitosan-Based Matrix.

A suite of four drug deliverance formulations grounded on 5-fluorouracil enclosed in a chitosan-founded intercellular substance was produced by 3,7-dimethyl-2,6-octadienal with in situ hydrogelation. The formulations have been examined from a morphological and structural point of view by Fourier transform infrared (FTIR) spectroscopy and microscopy with polarized light, respectively. The polarized optical microscopy (POM) pictures of the three representative formulations obtained were investigated by fractal analysis.

Antarctic aldehyde dehydrogenase from Flavobacterium PL002 as a potent catalyst for acetaldehyde determination in wine.

Latest solutions in biotechnologies and biosensing targeted cold-active extremozymes. Analysis of acetaldehyde as a relevant quality indicator of wine is one example of application that could benefit from using low-temperatures operating catalysts. In search of novel aldehyde dehydrogenases (ALDH) with high stability and activity at low temperatures, the recombinant S2-ALDH from the Antarctic Flavobacterium PL002 was obtained by cloning and expression in Escherichia coli BL21(DE3).

Time Estimation of Polymer Translocation through Nano-Membrane.

In this paper, the charged polymer escapement phenomenon, via a little hole of nano-metric dimensions arranged in a constitutive biological membrane, is studied. We will present the case of the transport process of an ideal polymer in a 3-dimensional extended region separated by a fine boundary named membrane in a free energy barrier attendance. Additionally, the general translocation time formula, respectively, the transition time from the cis area to the trans area, is presented.

Polymer Translocation through Nanometer Pores.

In this paper the loaded polymer transport and its escape via a nanometer size aperture, virtually by nanomembrane, the polymer being moved by an exterior electrostatic field, has been studied. Assuming a linear dependency of the friction coefficient on the number of segments and a parabolic behavior for the open-free (Gibbs) energy, in attendance of a present electrical potential across nanopore, an explicit flux formula for the polymers passed over a dimensional restricted pore, was derived. In addition, the linear polymers transport through a nanometer-sized pore under the action of a constant force is presented.

Fractal Modeling of Polymer Plasma Laser Ablation, Plasma Plume Tsallis Entropy and Its q-Statistics Interpretation, Part I: Theory.

Polymer plasma produced by laser ablation is investigated in a theoretical manner. In relation to the fact that the charge carrier circulation is assumed to take place on fractal curves, the so-called fractality type, electrical charge transport can be resolved by an extended scale relativity method. In addition, an elegant mathematical model, utilizing a conjecture of fractal space-time, is elaborated.

Fractal analysis in the quantification of medical imaging associated with multiple sclerosis pathology.

Backgrounds: Multiple sclerosis (MS) is an inveterate phlogistic situation characterized by focal and vaguely diffusive de-myelination and neurodegeneration, in the sphere of central nervous system (CNS). The brain's chronic inflammatory reaction includes astrocyte stimulation and microglial motivation, as well as macrophages marginal conscription. This lasting serious soreness of the brain is connected with neurodegeneration period and disability advance.

Microbiome pattern of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and feeding substrate in the presence of the foodborne pathogen Salmonella enterica.

The microbial diversity and quantitative dynamics during the insect's development stages constitute recently developed putative tools in forensic and medical studies. Meanwhile, little is known on the role of insects in spreading foodborne pathogenic bacteria and on the impact of these pathogens on the overall insects and feeding substrate microbiome composition. Here, we provide the first characterization of the bacterial communities harbored in adult and immature stages of Lucilia sericata, one of the first colonizers of decomposed human remains, in the presence of the foodborne pathogen Salmonella enterica using 16S rRNA Illumina sequencing and qPCR.

Electrical Signal Modeling in Cochlear Implants. Study of Temperature and Humidity Effects.

The present paper discusses the climatic effects of humidity and temperature on cochlear implant functioning and the quality of the electrical sound signal. MATLAB Simulink simulations were prepared, offering insights into signal behavior under such climatic parameter changes. A simulation setup of the cochlear implant was developed, where a source type selection was used to change between a voice recording and a "chirp" sound.

First report on antibiotic resistance and antimicrobial activity of bacterial isolates from 13,000-year old cave ice core.

Despite the unique physiology and metabolic pathways of microbiomes from cold environments providing key evolutionary insights and promising leads for discovering new bioactive compounds, cultivable bacteria entrapped in perennial ice from caves remained a largely unexplored life system. In this context, we obtained and characterized bacterial strains from 13,000-years old ice core of Scarisoara Ice Cave, providing first isolates from perennial ice accumulated in caves since Late Glacial, and first culture-based evidences of bacterial resistome and antimicrobial compounds production. The 68 bacterial isolates belonged to 4 phyla, 34 genera and 56 species, with 17 strains representing putative new taxa.

Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania.

Our understanding of the icy-habitat microbiome is likely limited by a lack of reliable data on microorganisms inhabiting underground ice that has accumulated inside caves. To characterize how environmental variation impacts cave ice microbial community structure, we determined the composition of total and potentially active bacterial communities along a 13,000-year-old ice core from Scarisoara cave (Romania) through 16S rRNA gene Illumina sequencing. An average of 2,546 prokaryotic gDNA operational taxonomic units (OTUs) and 585 cDNA OTUs were identified across the perennial cave ice block and analyzed in relation to the geochemical composition of ice layers.

Update on retinal vascular caliber.

Retinal vessels are the only blood vessels that can be viewed directly, in vivo, repetitively and non-invasively. Retinal vessel caliber is influenced by physiological (age, sex, race, blood pressure, blood glucose, body mass index) and pathological (atherosclerosis, dyslipidemia, smoking) determinants. There are studies on large population groups that demonstrate the associations between retinal vasculature and subclinical or clinical changes in systemic diseases (hypertension, diabetes, stroke, renal or cardiac diseases).

Pseudophakic bullous keratopathy.

Pseudophakic bullous keratopathy is characterized by corneal stromal edema with epithelial and subepithelial bullae due to cell loss and endothelial decompensation through trauma during cataract surgery. Patients present decreased vision, tearing, and pain caused by ruptured epithelial bullae. Cataract affects approximately 20 million people worldwide, and this complication can occur in 1 to 2% of the cataract surgeries.

Ocular posterior pole pathological modifications related to complicated pregnancy. A review.

Ocular posterior pole modification are a pathological manifestation in complicated pregnancies, especially when pregnancy induced hypertension is present (PIH), as well as in preeclampsia (PE) or eclampsia. Nonetheless, as the pregnancy evolves, the possibility for an aggravated evolution with HELLP syndrome, disseminated intravascular coagulation, and idiopathic thrombocytopenic purpura may have an ocular manifestation that, mainly, implies a loss of visual field or acuity, that, left unattended, may constitute a permanent impairment. Pregestational conditions like pituitary adenoma or genetic pedigree for complement factor H gene (1q31.

Effects of active versus passive recovery on thermoregulatory strain and performance in intermittent-sprint exercise.

Purpose: Team sports contain high-intensity sprints separated by active recovery (AR) and passive recovery (PR). The beneficial effects of AR on repeated-sprint performance, for short exercise duration, in thermoneutral environments, are well known. However, team sports are often performed in hot environments for prolonged periods.