In the frog genus , previous works found a sex-linked heteromorphism of the PcP190 satellite DNA in the nucleolus organizer region (NOR)-bearing chromosome pairs of and , which possess a ZZ/ZW sex determination system. A pericentromeric inversion was inferred to have occurred during W chromosome evolution, moving a chromosomal cluster enriched by the PcP190 from the short arm (as observed in ) to the NOR-bearing long arm (as observed in ). However, whether such an inversion happened in or in the common ancestor of and remained unclear.
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