Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect sp. as an Intermediary Host for Cotton Blue Disease.

The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (α-GQE, α-PRN, and α-INK) and monoclonal (α-GQE, α-PRN, and α-NKF) antibodies. All six were tested as capture antibodies, and polyclonal α-GQE and the monocle onal α-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm.

Genetic diversity and structure of natural populations of Gossypium mustelinum, a wild relative of cotton, in the basin of the De Contas River in Bahia, Brazil.

Gossypium mustelinum is a wild cotton relative found only in the semiarid region of Bahia state in Brazil, and changes caused by humans in the natural habitat of this species have endangered the existence of several natural populations. Information about the occurrence and genetic composition of these populations is necessary to design effective conservation measures. The aim of this study was to characterize the in situ maintenance mode and assess the genetic diversity of G.

Complete sequence of a new bipartite begomovirus infecting cotton plants in Brazil.

Begomoviruses are plant viruses responsible for severe losses in important crops, such as beans, cassavas, tomatoes, and cotton, around the world. Here, we report the first full-genome sequence of a bipartite begomovirus species collected from cotton plants in Brazil.

Meta-analysis of cotton fiber quality QTLs across diverse environments in a Gossypium hirsutum x G. barbadense RIL population.

Background: Cotton fibers (produced by Gossypium species) are the premier natural fibers for textile production. The two tetraploid species, G. barbadense (Gb) and G.