[The influence of periodontal diseases on the concentration of selected metals in saliva].

Analysis of elements (mainly metals) in biological materials provides a challenge for analytics. It results from complex matrix of this kind of samples and strict requirements for purity at all stages of the analytical process. Over the years many effective methods for determination of metals in body fluids have been developed, which link with searching for the association between elemental composition of human body and various diseases.

[Physiological metals in the serum, hair and nails of patients with head and neck cancer].

Cigarette smoking and excessive alcohol drinking result in the rise of numbers of patients suffering from the head and neck cancer. Addiction to any of these stimulants carry a risk of developing a cancerogenesis process. Using them simultaniously lead not to a summary of each of those risks but multiplies them.

Application of microwave irradiation to fast and efficient isolation of benzodiazepines from human hair.

The aim of this research was to develop, optimize and validate a modern and rapid method of preparation of human hair samples using microwave irradiation for the purpose of determination of six benzodiazepines: alprazolam, estazolam, lorazepam, clonazepam, diazepam and tetrazepam, with lormetazepam as an internal standard (IS). Extracts were analyzed ultrahigh performance liquid chromatography with mass spectrometry time of flight detection (UHPLC-MS-TOF). Optimal parameters of microwave-assisted extraction (type of extraction solvent, extraction temperature, extraction time and pH of borate buffer) for these drugs in spiked hair samples were defined.