Oviposition patterns of primary lepidopteran defoliators in soybean and the impact on structured refuge recommendations.

Background: Transgenic Bt technology in soybean, with plants expressing Cry1Ac, has been adopted as an insect pest management tool. It was first adopted in large areas of South America and Asia in 2013. The risk of resistance in target pests to this technology demands insect resistance management (IRM) programs.

Continental-scale migration patterns and origin of Helicoverpa zea (Lepidoptera: Noctuidae) based on a biogeochemical marker.

Insect migrations have ecological and economic impacts, particularly in agriculture. However, there is limited knowledge about the migratory movements of pests at the continental scale, which is an important factor influencing the spread of resistance genes. Understanding the migratory patterns of economic pests, like Helicoverpa zea (Boddie), is essential for improving Integrated Pest Management (IPM) and Insect Resistance Management (IRM) strategies.

The Spatiotemporal Distribution, Abundance, and Seasonal Dynamics of Cotton-Infesting Aphids in the Southern U.S.

Cotton leafroll dwarf virus (CLRDV) is an emerging aphid-borne pathogen infecting cotton, L., in the southern United States (U.S.

Extended Sentinel Monitoring of Resistance to Cry and Vip3Aa Toxins in Bt Sweet Corn: Assessing Changes in Phenotypic and Allele Frequencies of Resistance.

Transgenic corn and cotton that produce Cry and Vip3Aa toxins derived from (Bt) are widely planted in the United States to control lepidopteran pests. The sustainability of these Bt crops is threatened because the corn earworm/bollworm, (Boddie), is evolving a resistance to these toxins. Using Bt sweet corn as a sentinel plant to monitor the evolution of resistance, collaborators established 146 trials in twenty-five states and five Canadian provinces during 2020-2022.

Impact of seed blend and structured maize refuge on Helicoverpa zea (Lepidoptera: Noctuidae) potential phenological resistance development parameters in pupae and adults.

Background: Helicoverpa zea, an economic pest in the south-eastern United States, has evolved practical resistance to Bacillus thuringiensis (Bt) Cry toxins in maize and cotton. Insect resistance management (IRM) programs have historically required planting of structured non-Bt maize, but because of its low adoption, the use of seed blends has been considered. To generate knowledge on target pest biology and ecology to help improve IRM strategies, nine field trials were conducted in 2019 and 2020 in Florida, Georgia, North Carolina, and South Carolina to evaluate the impact of Bt (Cry1Ab + Cry1F or Cry1Ab + Cry1F + Vip3A) and non-Bt maize plants in blended and structured refuge treatments on H.

Flight Phenology of (Lepidoptera: Pyralidae) in the Northwest Florida Panhandle.

Zeller (Lepidoptera: Pyralidae), the lesser cornstalk borer (LCSB), is an economically important peanut pest in the southeastern U.S. region, and its occurrence and abundance have been associated with warm and dry conditions.

Magnitude and Extent of Resistance Levels to Cry1Ac and Cry2Ab2 across the Southeastern USA.

After resistance is first detected, continued resistance monitoring can inform decisions on how to effectively manage resistant populations. We monitored for resistance to Cry1Ac (2018 and 2019) and Cry2Ab2 (2019) from southeastern USA populations of . We collected larvae from various plant hosts, sib-mated the adults, and tested neonates using diet-overlay bioassays and compared them to susceptible populations for resistance estimates.

Ecology and management of the invasive land snail (Rafinesque, 1833) (Stylommatophora: Bulimulidae) in row crops.

Solutions for managing the growing populations of the snail (Rafinesque, 1833) in row crops, notably peanut ( L.), are urgently needed in the United States. This species has become a concern to the economy and food security for infesting commercial crops in U.

genus on the edge of the continental U.S.: Flight phenology, analysis of hybrid presence, and insecticide performance in high-input field crops in Puerto Rico.

The genus includes several agricultural pests globally. was reported in several countries in South America in 2013, and in Puerto Rico, in 2014. This territory is considered an agricultural hub, with a high-input system of seed production in the southern region of the island, and also at the edge of the continental U.

Pest population dynamics are related to a continental overwintering gradient.

Overwintering success is an important determinant of arthropod populations that must be considered as climate change continues to influence the spatiotemporal population dynamics of agricultural pests. Using a long-term monitoring database and biologically relevant overwintering zones, we modeled the annual and seasonal population dynamics of a common pest, (Boddie), based on three overwintering suitability zones throughout North America using four decades of soil temperatures: the southern range (able to persist through winter), transitional zone (uncertain overwintering survivorship), and northern limits (unable to survive winter). Our model indicates population dynamics are hierarchically structured with continental-level effects that are partitioned into three geographic zones.

Pyrethroid Susceptibility in Field Populations of Picture-Winged Flies (Diptera: Ulidiidae) Infesting Fresh Market Sweet Corn in Florida.

Picture-winged flies (Diptera: Ulidiidae) are the most damaging insect pests of sweet corn (Zea mays L.) produced in Florida for the fresh market. Management of these pests, referred to as corn silk flies, relies on frequent pyrethroid applications targeting adults.

(Hubner) (Lepidoptera: Noctuidae) Fitness and Resistance Stability to Diamide and Pyrethroid Insecticides in the United States.

In the United States, beet armyworm resistance to both chlorantraniliprole and bifenthrin insecticides was first reported in 2020. Here we examined beet armyworm fitness and stability of resistance to chlorantraniliprole and pyrethroid insecticides since knowledge of the stability of resistance is a crucial aspect when recommending rotation of insecticides with different mode of action. Concentration-mortality bioassays were performed with field and laboratory susceptible populations.

Seasonal Flight Patterns of Chrysodeixis includens (Lepidoptera: Noctuidae) in the Florida Panhandle and Inventory of Plusiine Species Cross-Attracted to Synthetic Pheromone.

Chysodeixis includens (Walker) is a polyphagous economic pest in agricultural landscapes. To detect the occurrence of this pest in the field, trapping using sex pheromone lures is often implemented. However, other plusiine species are cross-attracted to these lures and may be misidentified as C.

Extended investigation of field-evolved resistance of the corn earworm Helicoverpa zea(Lepidoptera: Noctuidae) to Bacillus thuringiensis Cry1A.105 and Cry2Ab2 proteins in thesoutheastern United States.

Previous studies have reported that the corn earworm/bollworm, Helicoverpa zea (Boddie), has developed field resistance to pyramided Bacillus thuringiensis (Bt) Cry1A/Cry2A maize and cotton in certain areas of the southeastern United States. The objective of the current study was to determine the current status and distribution of the resistance to Cry1A.105 and Cry2Ab2 in H.

Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in Brazil: the Big Outbreak Monitored by Light Traps.

The first detection of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in Brazil pointed to a gloomy future for South American agriculture in the 2012/2013 harvest, and insecticide sales increased exponentially during 2013 and 2014. However, after this outbreak, the population of this insect returned to manageable levels. This study documents data on H.

Populations of (Boddie) in the Southeastern United States are Commonly Resistant to Cry1Ab, but Still Susceptible to Vip3Aa20 Expressed in MIR 162 Corn.

The corn earworm, (Boddie), is a major pest targeted by pyramided (Bt) corn and cotton in the U.S. Cry1Ab is one of the first insecticidal toxins used in Bt crops, while Vip3A is a relatively new toxin that has recently been incorporated into Cry corn with event MIR 162 and Cry cotton varieties to generate pyramided Bt traits targeting lepidopteran pests including .

The Granulate Cutworm (Lepidoptera: Noctuidae): Biological Parameters Under Controlled Conditions, Host Plants, and Distribution in the Americas.

Feltia subterranea (Fabricius), commonly known as the granulate cutworm, is a common species of owlet moths (Noctuidae) of major agricultural importance, widely distributed in Nearctic and Neotropical regions. This study was conducted to determine the species biological parameters, gather information about its larval host plants, and assess the agricultural significance of this species in the Americas. The viability of the egg, larval, pupal stages, and prepupal period was 98, 98, and 100%, respectively, under laboratory conditions.

Demographic Performance of Populations on Dual and Triple-Gene Bt Cotton.

Insecticidal toxins from (Bt) are valuable tools for pest management worldwide, contributing to the management of human disease insect vectors and phytophagous insect pests of agriculture and forestry. Here, we report the effects of dual and triple Bt toxins expressed in transgenic cotton cultivars on the fitness and demographic performance of (Boddie)-a noctuid pest, known as cotton bollworm and corn earworm. Life-history traits were determined for individuals of three field populations from a region where overwintering is likely.

Role of nutritional composition in the development and survival of (Hübner) (Lepidoptera: Noctuidae) on artificial diet and natural hosts.

Helicoverpa armigera is a pest of several crops causing significant economic impact. We evaluated the insect development on different vegetative and reproductive structures of cotton, maize, and soybean compared to artificial diet. One hundred individuals were evaluated per structure (cotton leaves and bolls; maize leaves, grains, and silk; soybean leaves and pods) and artificial diet.

Contrasting susceptibility of lepidopteran pests to diamide and pyrethroid insecticides in a region of overwintering and migratory intersection.

Background: Pesticide resistance is a growing issue worldwide, and susceptibility of pest populations should be monitored in migratory intersection regions for successful resistance management. We determined the susceptibility of eight noctuid species from the Florida Panhandle to bifenthrin (pyrethroid) and chlorantraniliprole (diamide). Larvae from field and laboratory populations were exposed to commercial insecticide formulations using the leaf-dip method in concentration-mortality bioassays.

Adaptive Introgression across Semipermeable Species Boundaries between Local Helicoverpa zea and Invasive Helicoverpa armigera Moths.

Hybridization between invasive and native species has raised global concern, given the dramatic increase in species range shifts and pest outbreaks due to anthropogenic dispersal. Nevertheless, secondary contact between sister lineages of local and invasive species provides a natural laboratory to understand the factors that determine introgression and the maintenance or loss of species barriers. Here, we characterize the early evolutionary outcomes following secondary contact between invasive Helicoverpa armigera and native H.

Like Parents, Like Offspring? Susceptibility to Bt Toxins, Development on Dual-Gene Bt Cotton, and Parental Effect of Cry1Ac on a Nontarget Lepidopteran Pest.

An important step to devise appropriate pest management strategies for armyworms (Lepidoptera: Noctuidae) in Bacillus thuringiensis Berliner (Bt) crops is to determine the lethal, sublethal, and parental effects of Bt toxins on target and nontarget pest species. Here we documented the susceptibility of black armyworm, Spodoptera cosmioides (Walker), to three Cry toxins and its life-history traits feeding on dual-toxin Bt cotton and an artificial diet containing sublethal concentrations of Cry1Ac. In concentration-response bioassays, black armyworm larvae showed low susceptibility to Cry toxins, with 853 ng/cm2 as the lowest value estimated for the median lethal concentration (LC50).

Egg Albumin as a Protein Marker to Study Dispersal of Noctuidae in the Agroecosystem.

Knowledge of dispersal and spatial dynamics of pest populations is fundamental for implementation of integrated pest management and integrated resistance management. This study evaluated 1) the effectiveness of egg white albumin protein to mark larvae and adults of two polyphagous and highly mobile pests, Spodoptera frugiperda (J.E.