Publications by authors named "Paula T Del Giudice"
Purpose: To verify if the presence of varicocele (grades II and III) with and without seminal alterations, using the 5 centile cutoff values in table A1.1 of the World Health Organization (WHO, 2010) manual, alters the seminal plasma levels of proteins DNASE1 (deoxyribonuclease-1) and IGFBP7 (Insulin-like growth factor-binding protein 7), which are related to apoptosis regulation and cell proliferation, respectively, demonstrating that these proteins are important for correct spermatogenesis.
Methods: This cross sectional study was performed at the Sao Paulo Federal University Paulo between May 2014 and April 2016.
Purpose: Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation.
View Article and Find Full Text PDFObjective: To analyse the proteomic profile of seminal plasma with the aim of identifying the proteins and post-genomic pathways associated with sperm DNA fragmentation.
Materials And Methods: A cross-sectional study including 89 subjects from a human reproduction service was carried out. All semen samples were assessed for sperm DNA fragmentation using a comet assay.
Objective: To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI.
Design: Experimental study.
Setting: University hospital.
Objective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.
Design: Prospective study.
Setting: Academic research environment.
Endometriosis is a gynecological disease that affects women of reproductive age. The protein profiles of women with endometriosis who were able or unable to achieve pregnancy and women without endometriosis who did achieve pregnancy were compared in this study. The follicular fluid was collected from 21 patients undergoing in vitro-fertilization treatment, according to the following groups: nine women in the control group (Group C), four women with endometriosis who achieved pregnancy (Group E.
View Article and Find Full Text PDFObjective: To compare proteomic profiles of seminal plasma from adolescents with varicocele and changes in semen quality with the plasma from adolescents with varicocele without seminal changes and from adolescents without varicocele.
Design: Observational study.
Setting: Patients in an academic research environment.
Objective: To assess the effect of varicocelectomy on sperm function (DNA integrity and mitochondrial activity) and levels of lipid peroxidation in seminal plasma of adolescents.
Design: Prospective study.
Setting: Patients recruited from a local public school.
Objective: To evaluate the effects of cryopreservation on sperm mitochondrial activity and nuclear DNA integrity in men with spinal cord injury.
Design: Prospective controlled study.
Setting: Patients in an academic research environment.
Purpose: To assess the effect of leukocytospermia and semen processing on sperm DNA and mitochondria.
Methods: Twenty-two patients with and 41 without leukocytospermia were included. Sperm DNA fragmentation was assessed by the Comet assay, and mitochondrial activity by a colorimetric method for active mitochondria.