Publications by authors named "Paula Cabre Fernandez"
Microtubule associated protein 2 (MAP2) interacts with the regulatory protein 14-3-3ζ in a cAMP-dependent protein kinase (PKA) phosphorylation dependent manner. Using selective phosphorylation, calorimetry, nuclear magnetic resonance, chemical crosslinking, and X-ray crystallography, we characterized interactions of 14-3-3ζ with various binding regions of MAP2c. Although PKA phosphorylation increases the affinity of MAP2c for 14-3-3ζ in the proline rich region and C-terminal domain, unphosphorylated MAP2c also binds the dimeric 14-3-3ζ via its microtubule binding domain and variable central domain.
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- Rhabdomyosarcoma (RMS) treatment has improved, but high-risk patients still struggle with poor survival rates due to issues like PAX3/7-FOXO1 fusion proteins that cause metastasis and resistance to therapy.
- This study focused on TRIB3, a regulator of FOXO1, finding that higher TRIB3 expression in RMS tumors correlates with the fusion protein's presence and contributes to cell proliferation.
- Genetic inhibition of TRIB3 not only reduced PAX3-FOXO1 levels and affected signaling pathways but also delayed tumor growth and improved survival rates in vivo, indicating TRIB3 could be a promising therapeutic target for aggressive RMS.