Transitioning from static to suspension culture system for large-scale production of xeno-free extracellular vesicles derived from mesenchymal stromal cells.

Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) have shown increasing therapeutic potential in the last years. However, large production of EV is required for therapeutic purposes. Thereby, scaling up MSC cultivation in bioreactors is essential to allow culture parameters monitoring.

Biotransformation of (-)-cubebin by spp. into (-)-hinokinin and (-)-parabenzlactone, and their evaluation against oral pathogenic bacteria.

The biotransformation of the lignan (-)-cubebin by filamentous fungi and is an efficient bioprocess for obtaining (-)-hinokinin and (-)-parabenzlactone. The relevance of getting (-)-hinokinin is due to its promising effect against oral pathogens, especially (both MIC and MBC 12.5 μg/mL), and other previous reported effects against Chagas disease and as anti-inflammatory.

Culturing Drosophila melanogaster (S2) in a chemostat.

Insect cells are used for the expression of complex proteins in products such as vaccines and biopharmaceuticals. Physiology of a Drosophila melanogaster (lineage S2), transfected to stably express rabies virus glycoprotein (RVGP), was studied in batch culture and in a chemostat with serum-free medium. In batch mode, the system reached 3 × 10(7) cells mL(-1) with specific growth rate of 1.