Publications by authors named "Paul M Fox"
NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein , without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in , starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications.
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- Neural stem cells (NSCs) in the developing nervous system use temporal patterning to differentiate into various neuronal subtypes, with specific transcription factors guiding this process.
- A newly identified transcription factor named Chronophage (or 'time-eater') plays a crucial role in the transition from the Pdm to the Castor temporal windows, similar to mammalian CTIP1 and CTIP2.
- Chronophage not only promotes the shift to generating Castor-associated neurons but also represses earlier neuronal types, showcasing its importance in regulating NSC development and neuron specification.
Article Synopsis
- Temporal patterning in neural progenitors is a key evolutionary strategy for increasing diversity in neurons.
- Researchers developed a method called NanoDam to study how known temporal transcription factors relate to neuronal development in Drosophila.
- The study identified additional factors, Homeobrain and Scarecrow, that are important for defining specific temporal windows in neural development and have similar roles in the visual systems of other species.
Stem cells generate the differentiated progeny cells of adult tissues. Stem cells in the Caenorhabditis elegans hermaphrodite germline are maintained within a proliferative zone of ∼230 cells, ∼20 cell diameters in length, through GLP-1 Notch signaling. The distal tip cell caps the germline and supplies GLP-1-activating ligand, and the distal-most germ cells that occupy this niche are likely self-renewing stem cells with active GLP-1 signaling.
View Article and Find Full Text PDFThe C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cycling cells and cells in meiotic S phase, followed by entry into meiotic prophase.
View Article and Find Full Text PDFRhizobium sp. strain TAL1145 degrades the Leucaena toxin mimosine and its degradation product 3-hydroxy-4-pyridone (HP). The aim of this investigation is to characterize the Rhizobium genes for HP degradation and transport.
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