Spatial transcriptomics reveals alterations in perivascular macrophage lipid metabolism in the onset of Wooden Breast myopathy in broiler chickens.

This study aims to use spatial transcriptomics to characterize the cell-type-specific expression profile associated with the microscopic features observed in Wooden Breast myopathy. 1 cm muscle sample was dissected from the cranial part of the right pectoralis major muscle from three randomly sampled broiler chickens at 23 days post-hatch and processed with Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging and sequencing on the Illumina Nextseq 2000 system. WB classification was based on histopathologic features identified.

Effects of heat stress and a low energy diet on blood parameters and liver hsp70 and iNOS gene expressions in local chickens.

Two experiments were conducted to compare effects of heat stress and its combination with low dietary energy on blood indices, liver hsp70 and iNOS gene expressions in three Tanzanian local chicken ecotypes. In experiment one, five weeks old Kuchi (K), Ching'wekwe (C) and Morogoro medium (M) were randomly allocated to separate pens in a 3 × 2 factorial design in two adjacent rooms with controlled temperature. The study had three replicates consisting of 39 chickens per room, 13 per ecotype per pen making a total of 234 chickens.

Effects of "Bioactive" amino acids leucine, glutamate, arginine and tryptophan on feed intake and mRNA expression of relative neuropeptides in broiler chicks.

Feed intake control is vital to ensuring optimal nutrition and achieving full potential for growth and development in poultry. The aim of the present study was to investigate the effects of L-leucine, L-glutamate, L-tryptophan and L-arginine on feed intake and the mRNA expression levels of hypothalamic Neuropeptide involved in feed intake regulation in broiler chicks. Leucine, glutamate, tryptophan or arginine was intra-cerebroventricularly (ICV) administrated to 4d-old broiler chicks respectively and the feed intake were recorded at various time points.