Publications by authors named "Paul Kefer"
DNA damage reduces heterogeneity and coherence of chromatin motions.
Proc Natl Acad Sci U S A
July 2022
Article Synopsis
- Chromatin, which is the material that DNA is made of, moves around in different ways that can affect how our genes work, especially when DNA gets damaged.
- In yeast, when there are breaks in DNA, chromatin spreads out more, but in more complex organisms (like humans), it's more complicated.
- When DNA is damaged, the movement of chromatin changes; it slows down far from the damage but moves more freely near the break, which helps the cell to repair the damage and avoid problems with DNA structure.
Particle tracking in living systems requires low light exposure and short exposure times to avoid phototoxicity and photobleaching and to fully capture particle motion with high-speed imaging. Low-excitation light comes at the expense of tracking accuracy. Image restoration methods based on deep learning dramatically improve the signal-to-noise ratio in low-exposure data sets, qualitatively improving the images.
View Article and Find Full Text PDF