Numerical modeling of internal tides and submesoscale turbulence in the US Caribbean regional ocean.

The US Caribbean ocean circulation is governed by an influx of Atlantic water through the passages between Puerto Rico, Hispaniola and the Virgin Islands, and an interplay of the Caribbean Sea water with the local topography of the region. We present an analysis of the US Caribbean ocean flow simulated by the USCROMS; which is the ROMS AGRIF model configured for the US Caribbean regional ocean at a horizontal resolution of 2 km. Outputs from the USCROMS show a seasonal variability in the strength of submesoscale turbulence within a mixed layer whose depth varies from -70 to -20 m from winter to summer, and internal tides originating from the passages between the islands.

The visceral pericardium: macromolecular structure and contribution to passive mechanical properties of the left ventricle.

Much attention has been focused on the passive mechanical properties of the myocardium, which determines left ventricular (LV) diastolic mechanics, but the significance of the visceral pericardium (VP) has not been extensively studied. A unique en face three-dimensional volumetric view of the porcine VP was obtained using two-photon excitation fluorescence to detect elastin and backscattered second harmonic generation to detect collagen, in addition to standard light microscopy with histological staining. Below a layer of mesothelial cells, collagen and elastin fibers, extending several millimeters, form several distinct layers.

Limited utility of acetoxymethyl (AM)-based intracellular delivery systems, in vivo: interference by extracellular esterases.

The use of acetoxymethyl (AM) groups to deliver and trap exogenous optical probes inside cells is an established tool in cell biology/physiology, however, these probes have not been used extensively in vivo. In this study, the use of the acetoxymethyl delivery system for optical probes was evaluated, in vivo. Initial studies revealed very little trapped probe in intact tissues even when near saturating levels of probe were injected in living animals.

Skeletal muscle NAD(P)H two-photon fluorescence microscopy in vivo: topology and optical inner filters.

Two-photon excitation fluorescence microscopy (TPEFM) permits the investigation of the topology of intercellular events within living animals. TPEFM was used to monitor the distribution of mitochondrial reduced nicotinamide adenine dinucleotide (NAD(P)H) in murine skeletal muscle in vivo. NAD(P)H fluorescence emission was monitored ( approximately 460 nm) using 710-720 nm excitation.