Publications by authors named "Paul Gelfand"
Article Synopsis
- The study focuses on improving the production and purification efficiency of HIV-1 Envelope glycoprotein SOSIP trimers using transiently transfected ExpiCHO-S cells and the antibody PGT145.
- Researchers found that the PGT145 purification process was inconsistent and less effective with ExpiCHO-S supernatants compared to stable CHO cell lines or 293F supernatants.
- The presence of polyanions, particularly dextran sulfate in the transfection system, was identified as a major inhibitor of PGT145 binding, and alternative supplements were suggested to enhance trimer yields.
Fourier-transform infrared (FTIR) spectroscopic imaging is a widely used method for studying the chemistry of proteins, lipids, and DNA in biological systems without the need for additional tagging or labeling. This technique can be especially powerful for spatially resolved, temporal studies of dynamic changes such as in vivo protein folding in cell culture models. However, FTIR imaging experiments have typically been limited to dry samples as a result of the significant spectral overlap between water and the protein Amide I band centered at 1650 cm(-1).
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