Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants.

Objectives: To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants.

Results: A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains.

Conclusion: This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.

Retrograde popliteal approach to common femoral vein stenosis in an intravenous drug user with hostile groin: a case report.

Intravenous (IV) drug abuse is a common problem in our society. One complication of this practice is venous stenosis, endovascular management of which can be technically challenging especially in patients with a hostile groin. We describe an ipsilateral retrograde popliteal approach in a 26-year-old IV drug user presenting with swelling of the left leg secondary to common femoral vein stenosis.