High fidelity laser beam shaping using liquid crystal on silicon spatial light modulators as diffractive neural networks.

Spatial light modulators (SLMs) based on liquid crystal on silicon (LCoS) are powerful tools for laser beam shaping as they can be used to dynamically create almost arbitrary intensity distributions. However, laser beam shaping with LCoS-SLMs often suffers from beam shaping artifacts in part caused by unconsidered properties of the LCoS devices: astigmatism that stems from the non-normal incidence of the laser beam on the SLM and the effect commonly referred to as the '0-th diffraction order' that is caused by both the crosstalk between neighboring pixels and the direct reflection at the cover glass of the SLM. We here present a method to consider and compensate for these inherent properties of LCoS devices by treating the SLM as a diffractive neural network.

Advanced beam shaping for laser materials processing based on diffractive neural networks.

We propose a method based on neural network training algorithms for the design of diffractive neural networks - with the aim to perform advanced laser beam shaping in the NIR/VIS spectrum for laser materials processing. The method enables the efficient design of systems including multiple cascaded diffractive optical elements (DOEs) and allows the simultaneous optimization for complex (intensity and phase) target field distributions in multiple target planes. The multi-target boundary condition in the optimization method offers great potential for advanced laser beam shaping.

Freeform optics design for extended sources in paraxial approximation exploiting the expectation maximization algorithm.

Freeform optics generating specific irradiance distributions have been used in various applications for some time now. While most freeform optics design algorithms assume point sources or perfectly collimated light, the search for algorithms for non-idealized light sources with finite spatial as well as angular extent is still ongoing. In this work, such an approach is presented where the resulting irradiance distribution of a freeform optical surface is calculated as a superposition of pinhole images generated by points on the optical surface.

Attenuate Newcastle disease virus by codon modification of the glycoproteins and phosphoprotein genes.

A codon modification strategy was used to attenuate the avian pathogenicity of an oncolytic mesogenic Newcastle disease virus (NDV) by targeting the three major virulence factors: the fusion (F) protein, hemagglutinin neuraminidase (HN) and phosphoprotein (P). Recoding the F and HN genes with rare codons greatly reduced expression of both F and HN proteins and resulted in their low incorporation into virions. The F and HN recoded virus was partially attenuated in chickens even when the F protein cleavage site was modified.

A moonlighting enzyme links Escherichia coli cell size with central metabolism.

Growth rate and nutrient availability are the primary determinants of size in single-celled organisms: rapidly growing Escherichia coli cells are more than twice as large as their slow growing counterparts. Here we report the identification of the glucosyltransferase OpgH as a nutrient-dependent regulator of E. coli cell size.

Extreme C terminus of bacterial cytoskeletal protein FtsZ plays fundamental role in assembly independent of modulatory proteins.

Bacterial cell division typically requires assembly of the cytoskeletal protein FtsZ into a ring (Z-ring) at the nascent division site that serves as a foundation for assembly of the division apparatus. High resolution imaging suggests that the Z-ring consists of short, single-stranded polymers held together by lateral interactions. Several proteins implicated in stabilizing the Z-ring enhance lateral interactions between FtsZ polymers in vitro.

Actin-like protein 1 (ALP1) is a component of dynamic, high molecular weight complexes in Toxoplasma gondii.

Apicomplexan parasites, such as Toxoplasma gondii, rely on actin-based motility for cell invasion, yet conventional actin does not appear to be required for cell division in these parasites. Apicomplexans also contain a variety of actin-related proteins (Arps); however, most of these not directly orthologous to Arps in well-studied systems. We recently identified an apicomplexan-specific member of this family called Actin-Like Protein 1, (ALP1), which plays a role in the assembly of vesicular components recruited to the inner membrane complex (IMC) of daughter cells during cell division.