Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study.

Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs). This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation. CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs.

Measurements and modelling of the influence of dentine colour and enamel on tooth colour.

Objectives: We provide a quantitative predictive model for the extent to which coloured dentine, visible through the enamel, contributes to tooth colour. Our model uses (L(*),a(*),b(*)) measurements rather than spectral measurements.

Methods: We have used a model system, composed of a slice of bovine enamel placed on top of coloured paper.