Using diatom chain length as a bioindicator of heavy-metals contamination in marine environments.

The increasing release of toxic heavy metals into marine environments poses significant risks due to their persistence and bioaccumulation. Diatoms are ideal bioindicators because of their sensitivity to environmental changes. Despite traditional methods for detecting these persistent pollutants effectively identify composition and concentration, they are time-consuming, they often require the use of harmful reagents, and do not allow a fast assessment of detrimental impacts on marine organisms.

Toxic Effect of Metal Doping on Diatoms as Probed by Broadband Terahertz Time-Domain Spectroscopy.

The global marine environment is increasingly affected by human activities causing climate change, eutrophication, and pollution. These factors influence the metabolic mechanisms of phytoplankton species, such as diatoms. Among other pollutant agents, heavy metals can have dramatic effects on diatom viability.

Light-Induced Dynamic Holography.

Holographic photomobile polymers (H-PMP) are a novel class of photomobile materials in which holograms can be optically recorded. They can be used in a large variety of applications, including optical switches and color selectors. In this work, we show one of the most important properties of the photomobile film, which is the photophobicity of the unpolymerized parts of the photomobile mixture.

Service Utilization, Self-Efficacy, Positive Attitude and Well-Being Among Asian American Family Caregivers of Persons with Serious Mental Illnesses.

The well-being of the family caregivers of people with serious mental illnesses (SMIs) is a crucial success factor in the recovery of the afflicted family member. This cross-sectional quantitative study examined the associations between the use of formal mental health services, self-efficacy, and positive attitudes and their effects on the well-being of Asian American family caregivers of people with SMIs. Using convenience and snowball sampling, 116 Asian American family caregivers of people with SMIs in Northern California were recruited for this study.

Compact off-axis holographic slide microscope: design guidelines.

Holographic microscopes are emerging as suitable tools for diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through small and compact devices. In-line holographic microscopes can be realized at contained costs, trading off complexity in the phase retrieval process and being limited to sparse samples. Here we present a 3D printed, cost effective and field portable off-axis holographic microscope based on the concept of holographic microfluidic slide.

Long-term holographic phase-contrast time lapse reveals cytoplasmic circulation in dehydrating plant cells.

The intracellular dynamics of onion epidermal cells during the dehydration process is observed by holographic microscopy. Both the nucleus and cytoplasm are accurately revealed by quantitative phase imaging while dehydration takes place. Indeed, we notice that the contrast of phase images increases with the decrease in cellular water content.

Assembling and rotating erythrocyte aggregates by acoustofluidic pressure enabling full phase-contrast tomography.

The combined use of ultrasound radiation and microfluidics is a promising tool for aiding the development of lab-on-a-chip devices. In this study, we show that the rotation of linear aggregates of micro-particles can be achieved under the action of acoustic field pressure. This novel manipulation is investigated by tracking polystyrene beads of different sizes through the 3D imaging features of digital holography (DH).

Psychometric evaluation of the Heart Failure Somatic Perception Scale in a European heart failure population.

Background: The Heart Failure Somatic Perception Scale (HFSPS) is a four-factor instrument used to assess how bothersome are 18 physical signs and symptoms of heart failure. To date, construct validity and reliability of the HFSPS have been evaluated in only one American study and never in a European population.

Aim: To evaluate psychometric properties (validity and reliability) of the HFSPS in a European heart failure population.

Strategies for reducing speckle noise in digital holography.

Digital holography (DH) has emerged as one of the most effective coherent imaging technologies. The technological developments of digital sensors and optical elements have made DH the primary approach in several research fields, from quantitative phase imaging to optical metrology and 3D display technologies, to name a few. Like many other digital imaging techniques, DH must cope with the issue of speckle artifacts, due to the coherent nature of the required light sources.

Caregiver Contribution to Self-care in Patients With Heart Failure: A Qualitative Descriptive Study.

Background: Caregiver contribution to heart failure (HF) self-care maintenance and management is important in HF care. Literature remains unclear regarding which practices caregivers perform to contribute to self-care for patients with HF, especially in Southern Europe.

Objective: The objective of this study was to describe caregiver contributions to HF self-care maintenance (ie, treatment adherence and symptom monitoring) and management (ie, managing HF symptoms when they occur).

The Influence of Mutuality on Self-Care in Heart Failure Patients and Caregivers: A Dyadic Analysis.

Mutuality in patient-caregiver dyad may improve heart failure (HF) patient self-care and caregiver contribution to self-care, but literature is scarce. We evaluated the influence of mutuality and its dimensions on patient-caregiver dyad self-care. A sample of 366 HF patient-caregiver dyads was enrolled.

Rapid Prototyping of Plastic Lab-on-a-Chip by Femtosecond Laser Micromachining and Removable Insert Microinjection Molding.

We have introduced a new hybrid fabrication method for lab-on-a-chip devices through the combination of femtosecond laser micromachining and removable insert micro-injection molding. This method is particularly suited for the fast prototyping of new devices, while maintaining a competitive low cost. To demonstrate the effectiveness of our approach, we designed, fabricated, and tested a completely integrated flow cytometer coupled to a portable media device.

Endowing a plain fluidic chip with micro-optics: a holographic microscope slide.

Lab-on-a-Chip (LoC) devices are extremely promising in that they enable diagnostic functions at the point-of-care. Within this scope, an important goal is to design imaging schemes that can be used out of the laboratory. In this paper, we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup.

Resolution gain in space-time digital holography by self-assembling of the object frequencies.

Space-time digital holography (STDH) exploits the object motion to record the hologram in a hybrid ST domain. This representation adds new capabilities to conventional DH, e.g.

Psychometric characteristics of the mutuality scale in heart failure patients and caregivers.

Background: Higher patient-caregiver mutuality is associated with improved patient and caregiver outcomes, but no studies have tested the psychometric characteristics of the mutuality scale (MS) in heart failure (HF) patient and caregiver population.

Objectives: To test the validity and reliability of the MS.

Methods: A cross-sectional design.

Retrieving acoustic energy densities and local pressure amplitudes in microfluidics by holographic time-lapse imaging.

The development of techniques able to characterize and map the pressure field is crucial for the widespread use of acoustofluidic devices in biotechnology and lab-on-a-chip platforms. In fact, acoustofluidic devices are powerful tools for driving precise manipulation of microparticles and cells in microfluidics in non-contact modality. Here, we report a full and accurate characterization of the movement of particles subjected to acoustophoresis in a microfluidic environment by holographic imaging.

Patterns of self-care and clinical events in a cohort of adults with heart failure: 1 year follow-up.

Background: Heart failure (HF) self-care is important in reducing clinical events (all-cause mortality, emergency room visits and hospitalizations). HF self-care behaviors are multidimensional and include maintenance (i.e.

Psychometric evaluation of the Caregiver Preparedness Scale in caregivers of adults with heart failure.

Well-prepared informal caregivers play an important role in heart failure (HF) care, so an instrument to evaluate their preparedness to care is important. To date, HF caregiver preparedness has been rarely investigated quantitatively. The Caregiver Preparedness Scale (CPS) has been used in other chronic condition populations, but its psychometric characteristics have never been tested in HF caregivers.

Digital holography as 3D tracking tool for assessing acoustophoretic particle manipulation.

The integration of digital holography (DH) imaging and the acoustic manipulation of micro-particles in a microfluidic environment is investigated. The ability of DH to provide efficient 3D tracking of particles inside a microfluidic channel is exploited to measure the position of multiple objects moving under the effect of stationary ultrasound pressure fields. The axial displacement provides a direct verification of the numerically computed positions of the standing wave's node, while the particles' transversal movement highlights the presence of nodes in the planar direction.

Holographic microscope slide in a spatio-temporal imaging modality for reliable 3D cell counting.

In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LoC) microfluidic devices represent an innovative and cost-effective solution. In this framework, we propose for the first time a novel platform based on the compactness of a holographic microscope slide (HMS) in combination with the new computational features of space-time digital holography (STDH) that uses a 1D linear sensor array (LSA) instead of 2D CCD or CMOS cameras to respond to real diagnostic needs. In this LoC platform, computational methods, holography, and microfluidics are intertwined in order to provide an imaging system with a reduced amount of optical components and capability to achieve reliable cell counting even in the absence of very accurate flow control.

Caregiver determinants of patient clinical event risk in heart failure.

Background: Preventing hospitalization and improving event-free survival are primary goals of heart failure (HF) treatment according to current European Society of Cardiology guidelines; however, substantial uncertainty remains in our ability to predict risk and improve outcomes. Although caregivers often assist patients to manage their HF, little is known about their influence on clinical outcomes.

Aims: To quantify the influence of patient and caregiver characteristics on patient clinical event risk in HF.

The lived experience of caregivers of persons with heart failure: A phenomenological study.

Background: Heart failure (HF) patients need to follow a strict pharmacological and nonpharmacological regimen in order to counteract the burden of the disease, and informal caregivers are an important resource for HF patients in managing and coping with their disease. Few studies have examined the lived experience of these caregivers with a rigorous phenomenological approach, and none have been conducted in Italy.

Aim: To describe the lived experience of the caregivers of HF patients.

Psychometric characteristics of the caregiver burden inventory in caregivers of adults with heart failure.

Background: A better understanding of caregiver burden and its determinants is essential to support caregivers. Many instruments have been developed to measure caregiver burden in various illness contexts, but few have been psychometrically tested for caregivers of heart failure patients.

Aims: The aim of this study was to test the validity (factorial and concurrent validity) and reliability (internal consistency) of the caregiver burden inventory (CBI) in a cohort of caregivers of heart failure patients.

MOTIVATional intErviewing to improve self-care in Heart Failure patients (MOTIVATE-HF): Study protocol of a three-arm multicenter randomized controlled trial.

Aims: Heart Failure (HF) self-care improves patient outcomes but trials designed to improve HF self-care have shown inconsistent results. Interventions may be more effective in improving self-care if they mobilize support from providers, promote self-efficacy, increase understanding of HF, increase the family involvement, and are individualized. All of these elements are emphasized in motivational interviewing (MI); few trials have been conducted using MI in HF patients and rarely have caregivers been involved in MI interventions.

On-speckle suppression in IR digital holography.

Long-IR wavelength is the best option for capturing digital holograms of large-size, real-world objects. However, the coherent noise level in a long-IR hologram is by far larger than that of a visible wavelength recording, thus resulting in a poor quality of both numerical and optical reconstructions. In this Letter, we show how such coherent noise can be efficiently suppressed by employing an optical scanning multi-look approach, in combination with 3D block matching numerical filtering.